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一种快速、简便检测鸭乙型肝炎病毒抗原、抗体的方法
引用本文:张维,闻玉梅,李自力,罗玉芳.一种快速、简便检测鸭乙型肝炎病毒抗原、抗体的方法[J].复旦学报(医学版),1988(6).
作者姓名:张维  闻玉梅  李自力  罗玉芳
作者单位:上海医科大学基础医学部微生物学教研室 (张维,闻玉梅,李自力),上海医科大学基础医学部微生物学教研室(罗玉芳)
摘    要:本文报道检测鸭乙型肝炎表面抗原(DHBsAg)及抗体的斑点酶免疫测定法(Dot Euzyme Immunoassay,Dot EIA)。以硝基纤维素膜为载体依次加被检血清、抗DHBV免疫血清、辣根过氧化物酶标记葡萄球菌A蛋白(SPA-酶结合物),可测标本中DHBsAg。依次加纯化DHBV、被检血清、SPA-酶结合物则可测血清中抗体及效价。用此法检测自然感染鸭血清40份,实验感染鸭血清220份,并与DHBV核酸斑点杂交法比较分析,两种方法符合率94.2%。用本方法测定本室制备的兔抗DHBV免疫血清,抗体效阶为1/3200。Dot EIA简单、快速、敏感、特异,是研究DHBV较有实用价值的一种方法。

关 键 词:鸭乙型肝炎病毒  鸭乙型肝炎表面抗原  斑点酶免疫测定法

A SIMPLE RAPID METHOD FOR DUCK HEPATITIS B VIRUS ANTIGEN AND ANTIBODY ASSAY
Zhang Wei,Wen Yumei,Li Zhili,Luo Yufan.A SIMPLE RAPID METHOD FOR DUCK HEPATITIS B VIRUS ANTIGEN AND ANTIBODY ASSAY[J].Fudan University Journal of Medical Sciences,1988(6).
Authors:Zhang Wei  Wen Yumei  Li Zhili  Luo Yufan
Abstract:A dot enzyme immunoassay (dot EIA) for detecting DHBsAg and anti-DHBs is reported. Duck sera for assay of DHBsAg were spotted onto nitrocellulose filters dried and blocked. The filter membrane was then soaked in rabbit anti-DHBV serum, washed and incubated with peroxidase labeled staphylococcus protein A, followed by addition of 4-chloro-1-naphthol substrate. DHBsAg positive sera showed purple spots on the filter. "When purified DHBV were spotted, this test could be used to assay anti-DHBV. Compared to the DHBV DNA hybridization test among 260 sorum samples, the percentage of coincidence between the EIA and DHBV DNA hybridization tests was 94.2%. Rabbit anti-DHBV prepared in our lab showed an assay titer of 1:3 200. Dot EIA is not only rapid and simple but also specific and sensitive. It will be of practical use in lab for screening large numbers of duck serum samples without the need of sophisticated equipment.
Keywords:duck hepatitis B virus  duck hepatitis B surface antigen  dot enzyme immunoassay
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