首页 | 本学科首页   官方微博 | 高级检索  
检索        

骨保护素基因敲除小鼠发生骨质疏松症的特征
引用本文:陈子贤,盛哲津,邵云潮,施德源,陈统一.骨保护素基因敲除小鼠发生骨质疏松症的特征[J].复旦学报(医学版),2007,34(3):373-377.
作者姓名:陈子贤  盛哲津  邵云潮  施德源  陈统一
作者单位:1. 复旦大学附属中山医院骨科,上海,200032
2. 中国科学院上海生命科学研究院模式生物研究中心,上海,200031
摘    要:目的研究我国自行开发的骨保护素(OPG)基因敲除小鼠的骨质疏松基本数据及其所属的骨质疏松类型,为其进一步推广应用提供基线数据。方法将野生型OPG(OPG^-WT)小鼠,7和13周龄的骨保护素基因敲除小鼠(OPG^-/-)分成3组(每组6只),分别检测其血清碱性磷酸酶(ALP)、酸性磷酸酶(ACP)及骨钙素(OC)水平,并行骨密度测定、X线摄片、扫描电镜检查和生物力学测试,将所得数据进行统计学分析。结果OPG^-/-小鼠的骨密度、股骨刚度和最大折断负荷均显著降低(P〈0.01),血清ALP和ACP明显升高(P〈 0.01),OC在早期与对照组无明显差异。OPG^-/-小鼠在X线平片上表现为骨量减少,骨小梁稀疏或塌陷,骨皮质变薄。扫描电镜观察到OPG^-/-小鼠骨小梁变细、变尖,骨小梁间隙增大,部分骨小梁塌陷。结论OPG^-/-小鼠骨吸收活性增强伴继发性成骨活性提高,符合高转换型骨质疏松的特征,具有明显的优点,为研究OPG/OPGL/RANK体系提供了新的平台,有可能成为一种新的模型用于高转换型骨质疏松症的实验研究。

关 键 词:骨保护素  骨质疏松  基因敲除  动物模型
修稿时间:2006-10-16

Preliminary baseline observation of osteoporosis on osteoprotegerin-deficient mice
CHEN Zi-xian,SHENG Ze-jin,SHAO Yun-chao,SHI De-yuan,CHEN Tong-yi.Preliminary baseline observation of osteoporosis on osteoprotegerin-deficient mice[J].Fudan University Journal of Medical Sciences,2007,34(3):373-377.
Authors:CHEN Zi-xian  SHENG Ze-jin  SHAO Yun-chao  SHI De-yuan  CHEN Tong-yi
Institution:1. Department of Orthopaedics, Zhon gshan Hospital, Fudan University, Shanghai, 200032, China ; 2 Model Organism Research Center, Shanghai Institute for Biological Science, CAS, Shanghai, 200031 ,China
Abstract:Purpose To investigate the preliminary baseline data of osteoporosis on osteoprotegerin (OPG)-deficient mice. Methods Three groups of mice were included, i. e. , OPG-WT group (as a control), 7-week-old OPG gene knockout(OPG-/- ) group and 13-week-old OPG-/- group. Assessed parameters included serum alkaline phosphatase (ALP), acid phosphatase (ACP) and osteocalcin (OC), whole - body- radiography, BMD, biomechanical property of femurs; scan electron microscope was employed to observe the shape of lumbar vertebral trabecula. Results Compared with the control group, the level of serum ALP, ACP in both OPG-/- groups were significantly increased(P< 0.01), BMD, stiffness and strength of the femurs in OPG-/- groups were markedly decreased(P< 0.01). There was no significant difference in serum OC between the control group and 7-week-old OPG-/- group. Bone mass loss, cortical bone thinning, trabecular bone porosity and collapse were all found in radiographs of OPG-/- groups. Scan electron microscope showed that the vertebral trabeculae of OPG-/- groups were thin and discontinuous, with expanded trabecular space and partial collapse of trabeculae. Conclusions The osseous changes observed in OPG-/- mice are consistent with the characters of high- turnover osteoporosis, demonstrating a boost-up of bone resorption activity with secondary enhancement of bone formation, The OPG-/- mice may become a novel and-encouraging animal model for high turn-over osteoporosis, as well as for the study of the mechanisms of OPG/OPGL/ RANK system.
Keywords:osteoprotegerin  osteoporosis  gene knockout  animal model
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号