人血清类胰蛋白酶含量测定方法的建立

目的：检测正常人血清类胰蛋白酶含量，为肥大细胞相关疾病的诊断提供依据。方法：随机抽取正常人血清标本和1例过敏性休克死亡患者血清，用酶联免疫吸附试验(ELISA)法检测类胰蛋白酶的水平。结果：206份正常血清类胰蛋白酶含量的平均值为2.24ng/mL。过敏性休克死者血清胰蛋白酶含量为正常人的50倍。结论：本实验建立了较灵敏的检测类胰蛋白酶的ELISA法，为临床肥大细胞相关疾病的诊断提供依据。

Establishment of A New Experimental Detective Assay of Human Serum Tryptase

Purpose To detect normal human serum tryptase levels and to provide diagnoses supporting for mast cell associative diseases. Methods Mast cell tryptase was measured by the new enzyme-linked immunosorbent assay(ELISA) in 206 normal human sera and one post-mortem serum of anaphylactic shock. Results The mean levels of tryptase in serum from normal subjects from Shanghai is 2.24 ng/mL.The level of the post-mortem serum is fifty times than normal levels. Conclusions We established a ELISA which can detect tryptase sensitively and provide a base for clinical diagnosis of mast cell relating disease.