高脂血症小鼠心脏和肺组织中eNOS及HO-1的表达

 目的 观察高脂饲料喂养的小鼠心脏和肺组织中内皮型一氧化氮合酶（endothelial nitric-oxide synthase, eNOS）及血红素氧合酶-1（heme oxygenase-1, HO-1）的含量变化，探讨高脂血症对eNOS和HO-1表达的影响。方法 高脂饮食组（n=8）和普通饮食组（n=8）C57BL/6小鼠分别经高脂饲料和普通饲料喂养18周后，测血清总胆固醇（total cholesterol, TC）、甘油三酯（triglyceride, TG）、高密度脂蛋白（high density lipoprotein, HDL）和低密度脂蛋白（low density lipoprotein, LDL）含量，采用Real-Time PCR检测心脏和肺组织中eNOS及HO-1的mRNA水平，以免疫组化方法检测其蛋白表达。结果 高脂饮食组TC、TG和LDL明显高于普通饮食组，HDL则低于普通饮食组；高脂饮食组心脏和肺组织中eNOS的mRNA和蛋白水平低于普通饮食组，HO-1的mRNA和蛋白水平高于普通饮食组。结论 高脂饲料喂养能使小鼠形成高脂血症，并引起心脏和肺组织中eNOS的表达降低，同时HO-1的表达升高。

Expression of eNOS and HO-1 in heart and lung tissues of hyperlipidemia mice

Objective To explore the effects of hyperlipidemia in high-fat diet mice on expression of endothelial nitric-oxide synthase(eNOS)and heme oxygenase-1(HO-1)in heart and lung tissues.Methods C57BL/6 mice were fed a high-fat diet as high-fat diet group(n=8,high-fat diet group)and were fed a normal diet as control(n=8,normal diet group).Eighteen weeks later,serum lipid profiles including total cholesterol(TC),triglyceride(TG),high density lipoprotein(HDL)and low density lipoprotein(LDL)were detected.eNOS and HO-1 mRNA expression in heart and lung tissues were determined by Real-time PCR,as well as protein expression by immunohistochemistry.Results Levels of serum TC,TG and LDL significantly increased in high-fat diet group,while HDL decreased.mRNA and protein expression of eNOS in heart and lung tissues reduced in high-fat diet group,while HO-1 increased.Conclusions High-fat diet may lead to hyperlipidemia in mice,which may down-regulate eNOS expression and up-regulate HO-1 expression in heart and lung tissues.