CAPN4与食管鳞形细胞癌增殖和迁移关系的体外研究

 目的 探讨钙蛋白酶小亚基1(calpain small subunit 1,CAPN4)在食管鳞形细胞癌细胞系中的表达水平,分析其与食管鳞形细胞癌细胞增殖和迁移能力的相关性。 方法 挑选人正常食管上皮细胞系HEEC及食管鳞形细胞癌细胞系EC109与KYSE510,应用Western blot检测细胞系中CAPN4蛋白质水平,应用RNA干扰下调EC109与KYSE510中CAPN4的表达水平,通过CCK-8与Transwell实验分别检测细胞增殖与迁移能力的变化。同时用Western blot检测细胞系中增殖和迁移相关蛋白质水平。结果 CAPN4在EC109与KYSE510中的蛋白质水平显著高于HEEC,下调EC109与KYSE510中CAPN4蛋白质水平后,细胞增殖与迁移能力均显著降低(P<0.05),MMP-2蛋白质水平下调。 结论 CAPN4在食管鳞形细胞癌中高表达,促进食管癌增殖和迁移,并与下游分子MMP-2的表达水平密切相关。

In vitro study of the correlation between CAPN4 and the proliferation and migration in esophageal squamous cell carcinoma

Objective  To evaluate the protein level of Calpain small subunit 1 (CAPN4) in the cell lines of esophageal squamous cell carcinoma (ESCC),and the correlation between CAPN4 and ESCC cells＇s proliferation and migration. Methods  The human normal esophageal epithelial cell line HEEC and human ESCC cell lines EC109 and KYSE510 were picked up for experiments.Western blot was performed to detect the CAPN4 protein level in each cell line.Knockdown of CAPN4 expression was performed through the RNA interference.CCK-8 and Transwell were applied to compare the ability of proliferation and migration change after knockdown of CAPN4.Western blot was performed to detect the related protein level for proliferation and migration in each ESCC cell line.Results  CAPN4 protein level was upregulated in ESCC cell lines EC109 and KYSE510. Knockdown of the CAPN4 expression could inhibit cell proliferation and migration in vitro (P<0.05),and also downregulate the MMP-2 protein level.Conclusions  CAPN4 is upregulated in ESCC and promotes cancer cells proliferation and migration.CAPN4 is also significantly correlated with the expression of downstream MMP-2.