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分泌甲胎蛋白和乙型肝炎病毒表面抗原的肝癌细胞株的建立
引用本文:曹韵贞,顾桂玲,朝美玉,郑兆尧.分泌甲胎蛋白和乙型肝炎病毒表面抗原的肝癌细胞株的建立[J].复旦学报(医学版),1986(5).
作者姓名:曹韵贞  顾桂玲  朝美玉  郑兆尧
作者单位:上海医科大学中山医院免疫室 (曹韵贞,顾桂玲,朝美玉),上海医科大学中山医院肿瘤医院外科(郑兆尧)
摘    要:CZHC/8571细胞株来源于1例血清甲胎蛋白(AFP)和乙型肝炎表面抗原(HBsAg)阳性的肝细胞型肝癌伴慢性活动性肝炎患者。采用组织块培养法,9个月共传25代。从第10代开始进行生物学特性检测,其细胞形态和糖原染色经光学和电子显微镜观察均与原始癌细胞一致;细胞生长指数、分裂指数和倍增时间分别为7.9、70‰和64h;染色体主流数为84;C组染色体数目普遍增多,结构异常可见单、双体断裂、易位和环状染色体;浓缩20倍的24h培养液中即可测及AFP(对流法阳性);分别以5×10~6个细胞接种3只裸鼠,50~60d后均形成移植瘤。结果表明CZHC/8571细胞株是具有能产生和分泌AFP、HBsAg功能的人类肝癌细胞株。

关 键 词:肝肿瘤  细胞株  甲胎蛋白类  乙型肝炎表面抗原  肝炎  慢性活动性

ESTABLISHMENT OF AN AFP AND HBsAg-PRODUCING HUMAN HEPATOCELLULAR CARCINOMA CELL LINE
CAO YUNZHEN,Gu GUELING,Hu MEIYU.ESTABLISHMENT OF AN AFP AND HBsAg-PRODUCING HUMAN HEPATOCELLULAR CARCINOMA CELL LINE[J].Fudan University Journal of Medical Sciences,1986(5).
Authors:CAO YUNZHEN  Gu GUELING  Hu MEIYU
Abstract:A human hepatocellular carcinoma cell line designated as CZHO/8571 (Chinese Zhongshan Hepatocellular Carcinoma/8571) has been established by tissue fragment culture. It was derived from a case of hepatic carcinoma associated with chronic active hepatitis. The first transfer was oarriod out 32 days after culturing. The cells were maintained in continuous culture for 9 months (25 passages). Tho CZHC/8571 cell populations of the tenth transfer showed the following characteristics: (1) Morphologically the cells were distinctly similar to the original tumor cells; (2) their doubling time and mitotic indices were 64h and 70%, respectively; (3) their chromosome number varied widely, with a mode of 84; (4) AFP and HBsAg were detectable in the 20 times-concentrated cell culture medium after 24h of subculturing; (5) AFP and HBsAg-produoing tumors were induced in all 3 nude mice after inoculation with these cells.The results indicate that the CZHC/8571 cell line is a hepatocellular carcinoma cell line possessing the ability to synthesize and secrete AFP and HBsAg. It may serve as an experimental model in studies on the relationship between hepatitis B virus and hepatic carcinoma.
Keywords:liver neoplasms  cell line  alpha fetoproteins  hepatitis B surface antigens  hepatitis  chronic active  
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