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多西他赛对人肝癌放疗增敏及其作用机制探讨
引用本文:耿长新,曾昭冲,王吉耀.多西他赛对人肝癌放疗增敏及其作用机制探讨[J].复旦学报(医学版),2003,30(3):255-258.
作者姓名:耿长新  曾昭冲  王吉耀
作者单位:1. 复旦大学附属中山医院消化科,上海,200032
2. 复旦大学附属中山医院放疗科,上海,200032
摘    要:目的 观察不同浓度多西他赛(Docetaxel)在体外对人肝癌细胞的放射增敏作用及其机制。方法 用0.125、0.25、0.5nmol/L多西他赛作用人SMMC-7721肝癌细胞株24h和0.125、0.25nmol/L多西他赛作用48h后分别给予0、2,4、6、8、10Gy剂量照射,单纯照射组未给予多西他赛处理而接受同等剂量照射,用集落形成实验分析放射增敏效应。另外用流式细胞术检测上述不同浓度多西他赛分别作用不同时间SMMC-7721细胞内活性氧(reactive oxygen species,ROS)和用比色法测定谷胱甘肽(glutathione,GSH)含量的变化。结果 用0.125、0.25、0.5nmol/L多西他赛作用24h和0.125、0.25nmol/L多西他赛作用48h均能观察到各自的放射增敏作用,其放射增敏比(SERDq)分别为1.12、1.19、1.43和1.37、1.48。同时观察到上述剂量多西他赛作用不同时间导致SMMC-7721细胞内ROS产生增多和GSH降低。结论 上述剂量多西他赛在体外对SMMC-7721肝癌细胞具有放射增敏作用,其增敏机制与用药后细胞内ROS产生增多和GSH降低有关。

关 键 词:多西他赛  放射增敏作用  ROS  GSH  剂量  观察  SMMC-7721细胞  活性氧  谷胱甘肽  照射
修稿时间:2002年10月15

Docetaxel Shows Radiosenstization in SMMC-7721 Human Hepatocellular Carcinoma Cells
GENG Chang-xin,ZENG Zhao-chong,WANG Ji-yao.Docetaxel Shows Radiosenstization in SMMC-7721 Human Hepatocellular Carcinoma Cells[J].Fudan University Journal of Medical Sciences,2003,30(3):255-258.
Authors:GENG Chang-xin  ZENG Zhao-chong  WANG Ji-yao
Abstract:Purpose To determine the radiosensitizing potential of docetaxel in human hepatocellular car-cinoma(HCC) SMMC-7721 cells. Methods SMMC-7721 cells were incubated with docetaxel at 0.125, 0.25,0.5 nmol/L for 24 hr and at 0.125,0.25 nmol/L for 48 hr before irradiation. Radiation doses were given from 0 to 10 Gy. Cell survival was measured by a standard clonogenic assay after a 9 day incubation. The reactive oxygen species( ROS) and glutathione( GSH) were detected after the cells were given the same dose of docetaxel for the same time. Results The sensitization enhancement ratios(SERDq) for SMMC-7721 cells determined were 1.12,1.19,1.43 at 0.125,0.25,0.5 nmol/L for pre-incubation 24 hr, respectively; the SERDq, were 1. 37,1. 48 at 0. 125,0. 25 nmol/L for pre-incubation 48 hr, respectively. The SMMC-7721 cells ROS increased and GSH decreased after pretreatment with the same doses of docetaxel for 24 hr or 48 hr. Conclusions A radiosensitizing effect of docetaxel could be demonstrated unambiguously in this cell line used. In addition,our data show that the mechanism of radiopotentiation by docetaxel probably does not involve a G2/M block in SMMC-7721 cells,and ROS generation and GSH deletion may play a key role in the radiosensitizing effect of docetaxel.
Keywords:docetaxel  hepatocellular carcinoma  SMMC-7721 cell line  radiosensitization  reactive oxygen species  glutathione
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