风心瓣膜病变患者红细胞膜钠、钙泵活性和脂质过氧化的变化

用酶解法和Ｈ２Ｏ２分解法分别测定２８例风心瓣膜病变患者红细胞膜钠泵（Ｎａ＋、Ｋ＋－ＡＴＰ酶）、钙泵（Ｃａ２＋、Ｍｇ２＋－ＡＴＰ酶）活性和膜过氧化氢酶（ＣＡＴ）活性，并用硫代巴比妥酸比色法测定患者红细胞膜脂质过氧化物（ＬＰＯ）含量。患者红细胞膜Ｎａ＋、Ｋ＋－ＡＴＰ酶活性和Ｃａ２＋、Ｍｇ２＋－ＡＴＰ酶活性分别显著低于正常人３４．７５％（Ｐ＜０．０１）和２６．７７％（Ｐ＜０．０１）；ＣＡＴ活性显著低于正常人２０．０７％（Ｐ＜０．０１）；而患者ＬＰＯ含量却显著高于正常人９７．５０％（Ｐ＜０．０１）。结果提示风心病患者瓣膜病变与质膜钠、钙泵活性变化以及膜脂质过氧化作用有关。

CHANGES OF THE ACTIVITIES OF ERYTHROCYTE MEMBRANE CALCIUM PUMP, SODIUM PUMP AND LIPID PEROXIDATION IN RHEUMATIC HEART VAVLE DISEASE PATIENTS

The activities of the erythrocyte membrane calcium pump (Ca2+,Mg2+-ATPase) and sodium pump(Na+,K+-ATPase) were determined with the aid of ammonium molybdate method. the membrane catalase(CAT) activity was measured by dynamic method,and the content of lipid peroxide(LPO) was assayed by the use of spectrophotometry of thiobarbituric acid in rheumatic heart vavle disease patients and normal subjects. The activities of Na+ ,K+-ATPase and Ca2+, Mg2+-ATPase in rheumatic heart disease patients were 34.75% and 26.77% lower than those in the normal respectively (P<0.01,n=40).The CAT activity in rheumatic heart vavle disease patients was 20.07% lower than that in the normal (P<0. 01,n=28).However,the content of LPO was 97.50% higher than that in the normal (P<0. 01,n=28).The results indicate that the heart valve injury may be related to the activities of membrane Ca2+,Mg2+-TAPase,Na+,K+-ATPase and CAT,and the lipid peroxidation in rheumatic heart vavle disease patients.