疏肝健脾活血方对肝星状细胞增殖的影响

目的:采用细胞培养的方法,体外观察疏肝健脾活血方药物血清对转化生长因子β1(transforming growth factor,TGFβ1)诱导的大鼠肝星状细胞(hepatic stellate cell,HSC-T6)增殖作用的影响。方法:将40只大鼠随机分成正常大鼠血清组,疏肝健脾活血方低剂量组,疏肝健脾活血方中剂量组,疏肝健脾活血方高剂量组。以疏肝健脾活血方给大鼠灌胃,腹主动脉采血并制备血清。采用TGFβ1诱导HSC-T6作为活化的肝星状细胞的研究模型,以不同浓度的疏肝健脾活血方药物血清作用于体外培养的HSC-T6,分别作用24 h、48 h后,采用CCK-8法检测不同浓度的疏肝健脾活血方药物血清对HSC-T6增殖的影响。结果:疏肝健脾活血方药物血清作用于TGFβ1诱导的HSC-T6,细胞增殖受到明显抑制。各组作用48 h后,空白对照组OD值为(0.617±0.061),疏肝健脾活血方低剂量组、中剂量组、高剂量组OD值分别为(0.446±0.091)、0.417±0.103)、(0.376±0.065),疏肝健脾活血方各剂量组与空白组比较,差异均有统计学意义(P0.05)。正常大鼠含药血清组OD值为(0.506±0.015),与空白对照组比较,差异无统计学意义(P0.05)。结论:疏肝健脾活血方药物血清对TGFβ1诱导的HSC-T6细胞活化作用增殖有抑制作用。

The Effect of Shugan Jianpi Huoxue Decoction on Hepatic Stellate Cell Proliferation

Objective:To explore the influence of proliferative effects of Shugan Jianpi Huoxue Decoction drug serum on hepatic stellate cells (HSC-T6) induced by transforming growth factor 1 (TGFβ1).Methods:40 rats were randomly divided into normal rat serum group;Shugan Jianpi Huoxue Decoction in low,middle and high concentration group.Rats were given intragastric administration of Shugan Jianpi Huoxue Decoction,abdominal aortic blood serum and preparation.Taking TGFβ1 induced activated HSC-T6 cells as research model,with different concentrations of Shugan Jianpi Huoxue Decoction drug serum,the influence of proliferative effects of Shugan Jianpi Huoxue Decoction drug serum on HSC-T6 were studied by CCK-8 assay at 24 and 48 hours,respectively.Results:The Shugan Jianpi Huoxue Decoction drug serum had inhibitory effect,in time and concentration dependent,on TGFβ1 induced HSC-T6 cells.Compared with blank control group,the values of A of different groups whose cells were treated by different concentrations of drug sera at low (0.446 ±0.091),middle(0.417 ±0.103) and high (0.376 ±0.065),especially 48 h later,there were significant differences in each concentration group (P ＜ 0.05).showed significantly difference (P ＜ 0.05).The values of A of normal rat serum group A was (0.506 ± 0.015),compared with the control group,the difference was not statistically significant (P ＞ 0.05).Conclusion:The Shugan Jianpi Huoxue Decoction drug serum has certain inhibitory effect on proliferative effect of TGFβ1 induced activated HSC-T6 cells.