原花青素预处理对大鼠心肌缺血再灌注损伤的作用

目的:探讨原花青素(proanthocyanidins,PC)对大鼠缺血再灌注损伤(ischemia reperfusion injury,IRI)心肌能量代谢的影响及其抗氧化能力。方法:雄性Wistar大鼠40只,随机均分为4组:空白对照组、IRI模型组、PC 80 mg·kg~(-1)、PC160 mg·kg~(-1)组。首先用不同剂量的PC对大鼠进行预处理,然后利用Langendorff灌流技术制备心肌IRI模型,并观察PC作用后,IRI心肌高能磷酸化合物(high energy phosphates,HEP)、超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)的变化。结果:PC 160 mg·kg~(-1)组和PC 80 mg·kg~(-1)组左心室心肌组织的三磷酸腺苷(adenosine triphosphate,ATP)、二磷酸腺苷(adenosine diphosphate,ADP)、一磷酸腺苷(adenosine monophosphate,AMP)及总腺嘌呤核苷酸(total adenine nucleotides,TAN)分别为(0.16±0.06)mg·g~(-1),(0.51±0.15)mg·g~(-1),(0.80±0.26)mg·g~(-1),(1.47±0.44)mg·g~(-1)和(0.14±0.07)mg·g~(-1),(0.34±0.11)mg·g~(-1),(0.74±0.31)mg·g~(-1),(1.22±0.44)mg·g~(-1),均高于IRI模型组(P0.01);PC 160 mg·kg~(-1)组左心室肌组织的ADP高于PC 80 mg·kg~(-1)组(P0.01)。与IRI模型组比较,PC 2个剂量组左心室肌组织的SOD活性分别为(154.3±23.7)n U·mg~(-1),(134.1±18.8)n U·mg~(-1),活性升高(P0.01,P0.05);MDA含量分别为(3.4±0.6)μmol·g~(-1),(2.6±0.6)μmol·g~(-1),含量减少(P0.01);PC 2个剂量组左心室肌组织的SOD活性、MDA含量比较,差异均有统计学意义(P0.05)。结论:PC具有改善IRI心肌能量代谢和抗氧化的作用,能起到保护心肌IRI的作用。

Effect of Proanthocyanidins on Myocardial Ischemia/Reperfusion Injury in Rats

Objective:To observe the effects of proanthocyanidins (PC) on the energy metabolism of myocardium with ischemia/ reperfusion injury (IRI) and the antioxidation.Methods:Forty Wistar rats were randomly divided into 4 groups:the control group,the myocardial IRI model group,the two PC groups with the dose of 160 mg · L-1 and 80 mg · L-1,with 10 rats in each group.All the rats in the two groups were given with different PC.After the handling,a myocardial IRI model was established with the Langendorff method.In each group,the high energy phosphates (HEP) concentrations,superoxide dismutase (SOD) and malondialdehyde(MDA) were determined by HPLC in myocardium of all the 10 rats.Resuhs:The adenosine triphosphate (ATP),adenosine diphosphate(ADP),adenosine monophosphate (AMP) and total adenine nucleotides (TAN) levels of the PC 160 mg · kg-1 group and PC 80 mg · kg-1 group were 0.16 ± 0.06 mg · g-1,0.51 ± 0.15 mg · g-1,0.80 ± 0.26 mg · g-1,1.47 ± 0.44 mg · g-1 and 0.14 ± 0.07 mg · g-1,0.34 ± 0.11 mg · g-1,0.74 ± 0.31 mg · g-1,1.22 ± 0.44 mg · g-1.Compared with the myocardial IRI model group,they were increased significantly in the two PC groups (P ＜ 0.01).The ADP level of the PC 160 mg · kg-1 group was higher than that of the PC 80 mg · kg-1 group(P ＜ 0.01).The SOD levels of these two groups were 154.3 ± 23.7 NU · mg-1,134.1 ± 18.8 NU · mg-1,and the MDA levels of these two groups were 3.4 ±0.6 nmol · mg-1,2.6 ±0.6 nmol · mg-1.Compared with the myocardial IRI model group,SOD levels was increased significantly in the two PC groups (P ＜ 0.01,P ＜ 0.05),and MDA levels was decreased significantly in the two PC groups(P ＜ 0.01).The SOD level of the PC 160 mg · kg-1 group was higher than that of the PC 80 mg · kg-1 group(P ＜0.05),and the MDA level of the PC 160 mg · kg-1 group were lower than that of PC 80 mg · kg-1 group(P ＜ 0.05).Conclusion:PC can improve the energy metabolism of myocardium with isehemia/reperfusion injury,and has the antioxidation.