老年人群血管内皮型一氧化氮和酶基因Glu298Asp多态性与原发性高血压病的关系

目的:了解中国老年人群血管内皮型一氧化氮合酶(eNOS)基因Glu298Asp多态性的分布,探讨其与原发性高血压病的关系。方法:随机选择门诊及住院病例中确诊的原发性高血压病患者95例为研究组,同时选择性别、年龄与研究组相匹配的体检正常者95例作为对照组,分别测定他们的空腹血脂、身高、体重及坐位血压,应用聚合酶链反应(PCR)和限制性片断长度多态性分析(RELP)检测eNOS基因Glu298Asp多态性,并进行两组间的比较。结果:研究组Glu298Asp基因型构成比为26.3％,对照组为12.6％,两组eNOS基因Glu298Asp构成间差别有显著性意义(Z2=5.67,P<0.05)。298Asp等位基因频率研究组为13.2％,对照组为6.3％,两组等位基因频率间差别亦有显著性意义(Z2=5.06,P<0.05)。结论:eNOS基因Glu298Asp变量可能是中国人原发性高血压病的一种遗传易感指针。

Association of Glu298Asp Polymorphism of the Endothelial Nitric Oxide Synthase Gene with Essential Hypertension in Elderly People

Objective: To investigate the association of Glu298Asp polymorphism of the eNOS gene with essential hypertension in elderly people. Methods: Ninety-five cases of essential hypertension were randomly chosen from outpatients and inpatients as the study group, and an equal number of sexes,age-matched healthy people as the control group. Their height,weight and blood pressure were recorded and tlieir fasting plasma lipid concentrations were measured. Glu298Asp polymorphism of the eNOS gene was measured using the methods of PCR and RFLP. Results: The constituent ratio of Genotype Glu/Asp in the study group (26. 3%) was higher than that in the control group (12. 6 % , X2 = 5. 67,P<0. 05),the allelic frequency of 298Asp in the study group (13. 2%) was significantly higher than that in the control group (6. 3%,X2 = 5. 06,P<0. 05). Conclusion: Glu298 Asp variant of the eNOS gene may be an independent predictor in essential hypertension.