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Chrysanthemum indicum linné extracts attenuate the mitogenic effect of epinephrine on human HCC cells
Authors:Aihua Yuan  Zongfang Li  Xinqiu Li  Shukui Wang  Jianmin Bian
Institution:a Department of General Surgery, Second Affiliated Hospital of Medical College of Xi' an Jiaotong University, Xi' an, China
b Department of General Surgery, Nanjing First Hospital Affiliated to Nanjing Medical University, Nanjing, China
c Department of Hepatobiliary Surgery, First Affiliated Hospital of Medical College of Xi' an Jiaotong University, Xi' an, China
d Department of Cancer Center, First Affiliated Hospital of Medical College of Xi' an Jiaotong University, Xi' an, China
Abstract:

Objective

To investigate the stimulatory effect of epinephrine(Epi) and the antagonistic effect of Chrysanthemum indicum Linné extracts (CILE) on Epi-induced growth of human hepatocellular carcinoma(HCC) cells.

Methods

The stimulatory effect of Epi and inhibitory effect of CILE on the growth of HepG2 and MHCC97H cells were investigated using a proliferation assay in correlation with β adrenergic receptor(β2-AR) blockade, a MEK1/MEK2 inhibitor, and assessment of MAPK/ERK½ intracellular activity.

Results

Epi transiently activated MAPK/ERK½ in HepG2 and MHCC97H cells, resulting in a burst of growth. The effect of Epi was significantly attenuated by ICI 118551 and U0126. CILE exhibited a dose-dependent attenuation of the stimulatory effect of Epi on the growth of both cell lines and inhibited the Epi-induced activation of MAPK/ERK½.

Conclusion

Epi, mimicking a mitogen, stimulated the growth of HepG2 and MHCC97H cells, and CILE was effective in attenuating this effect of Epi on tumor cells by inhibiting the β2-AR-mediated activation of MAPK/ERK½.
Keywords:Chrysanthemum indicum Linné  extracts  epinephrine  extracellular signal-regulated kinase ½    hepatocellular carcinoma
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