TNF-α在大鼠重症急性胰腺炎并发肾损伤时的作用

目的探讨重症急性胰腺炎并发急性肾损伤时TNF-α，RNA表达变化及其机制。方法32只健康SD大鼠随机分为两组：假手术组（n=16）、胰腺炎组（n=16）。假手术组开腹仅翻动肠管；胰腺炎组经胆胰管逆行注射3％牛磺胆酸钠（0．05ml/kg）建立大鼠SAP模型。分别于制模后12、24h抽血测定血清淀粉酶活性，血清尿素氮、肌酐浓度和肾脏组织TNF-α mRNA表达变化，观察胰腺及肾脏病理形态学改变。结果胰腺炎组大鼠血清淀粉酶活性，血清尿素氮、肌酐浓度及肾组织TNF-α mRNA表达水平均较假手术组明显升高。胰腺及肾脏病理损害严重，腹水的生成和皂化斑的形成多；假手术组未见明显异常。结论SD大鼠SAP时肾组织TNF-α mRNA表达水平明显升高，提示肾脏组织TNF-α在SAP合并ARI的发病机制中可能具有重要作用。

Experimental study of the TNF-α of Rats in severe acute pancreatitis with acute Renal Injury

Objective To explore the expressive changes of TNF-amRNA in severe acute pancreatitis complicated with acute Renal Injury （ARI） and its mechanisms. Methods 32 healthy SD rats were randomly divided into two groups：sham operation group （n=16）,pancreatitis model group （n=16）. sham operation group： open rats abdomen and only tipping intestinal canal;the model of severe acute pancreatitis was induced by retrograde injection of 3% Sodium Taurocholate into bilio-pancreatic duct. All rats were sacrificed after the SAP model had been produced at 12 hours and 24 hours .The rats blood was drawn from portal vein and detected the Concentrations of blood serum - amylase,blood serum-urea nitrogen, creatinine and the expression of renal tissue TNF-α mRNA. Pathologic change of kidney and pancreas were observed under light microscope. Results The concentrations of blood serum amylase,blood serum -urea nitrogen,creatinine and the expression of renal tissue TNF-α mRNA were higher in pancreatitis Group than that in sham operation Group. pathological lesion were server in pancreatitis Group, ascetic and specifications were emerged in pancreatitis Group. sham operation group was normal. Conclusions The expression of renal tissue TNF-α mRNA and alleviate the pathologic damage of the pancreas and kidney in rats SAP. it is a cute that the TNF-α mRNA of renal tissue play a very important etiopathogenisis role in rats SAP complicated with ARI.