| 女贞属苦丁茶中总黄酮的含量测定 |
| |
| 引用本文： | 张倩茹,南莹,娄方明,黄燮南.女贞属苦丁茶中总黄酮的含量测定[J].遵义医学院学报,2011,34(1):79-81. |
| |
| 作者姓名： | 张倩茹 南莹 娄方明 黄燮南 |
| |
| 作者单位： | 遵义医学院药学院,贵州遵义,563000 |
| |
| 基金项目： | 贵州省卫生厅项目，遵义市红花岗区科技局项目，遵义医学院立项项目 |
| |
| 摘 要： | 目的测定贵州不同产地苦丁茶总黄酮化合物的含量。方法 L9（34）正交试验优选提取条件,用比色法测定苦丁茶中总黄酮化合物含量。结果最佳提取条件为：用60倍90%乙醇提取苦丁茶粗粉2次,每次1h,用比色法测定总黄酮化合物的含量。芦丁标准品在0.4868-2.434mg浓度范围线性关系良好,线性方程为：y=0.3139x-0.0062,r=0.9960。平均回收率为98.77%,RSD为1.5%。贵州多个产地的苦丁茶中总黄酮的含量均在30%以上。结论贵州不同产地的苦丁茶中总黄酮的含量较高且存在差异,本方法方便,可靠,重复性好,可用于苦丁茶中总黄酮的含量测定。
|
| 关 键 词： | 苦丁茶 女贞属 总黄酮 比色法 |
The content determination of flavonoids in Ligustrum Kudingcha |
| |
| ZHANG Qianru,NAN Ying,LOU Fang-ming,HUANG Xie-nan.The content determination of flavonoids in Ligustrum Kudingcha[J].Acta Academiae Medicine Zunyi,2011,34(1):79-81. |
| |
| Authors: | ZHANG Qianru NAN Ying LOU Fang-ming HUANG Xie-nan |
| |
| Institution: | ZHANG Qianru,NAN Ying,LOU Fang-ming,HUANG Xie-nan(Pharmacy department,Zunyi Medical College,Guizhou Zunyi 563000) |
| |
| Abstract: | Objective To determinate the content of total flavonoids in Ligustrum Kudingcha from different areas of Guizhou Provice.Methods L9(34) orthogomnal test was used to optimize the best extract conditions of flavonoid in Kudingcha,and the contents were detected by colorimetry.Results The optimum extracting factors were 60 times of 90% alcohol,refluxing and extracting for 1h,for 2 times.Lutin had a good linear relationship at the range of 0.4868~2.434mg/ml,the linear equation was: y=0.3139x-0.0062,r=0.9960,avera... |
| |
| Keywords: | kudingcha ligustrum flavonoids colorimetry |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
