基于NMR代谢组学方法研究高果糖诱导大鼠肝脏组织的代谢变化

目的应用基于核磁共振氢谱代谢组学方法研究高果糖喂养大鼠肝脏组织的代谢变化。方法Wistar大鼠随机分为对照组和模型组，每组8只。对照组给予纯净水饲养；模型组给予10％果糖水饲养．制备非酒精性脂肪肝模型。实验8周后取大鼠肝脏组织，采集两组大鼠肝脏组织水溶性提取液的’HNMR谱，运用主成分分析法（principal component analysis，PCA）分析。结果与对照组相比，模型组中乳酸、肌酸和牛磺酸升高，异亮氨酸、亮氨酸、谷氨酰胺、胆碱和甜菜碱／氧化三甲胺降低，差异均有统计学意义。结论应用核磁共振和模式识别的代谢组学方法，获得高果糖喂养大鼠的肝脏组织提取液的代谢特征，为理解高果糖引起肝脏脂肪病变过程中的代谢变化提供了依据。

1H NMR metabonomics study of liver extracts from fructose-fed rats

Objective To study the metabolic changes of liver extracts from fructose-fed rats with nuclear magnetic resonance 1H spectroscopy. Methods 16 Wistar rats were randomly divided into two groups, namely the control and model groups. The model group was given 10% fructose water to establish the non-alcoholic fatty liver disease （NAFLD）. The control group was given pure water with equal volume during the period. After 8 weeks, liver tissues were collected and ^1H NMR spectra of each sample extract was analyzed by principal component analysis （PCA） method.Results Compared with the control rats,lactate, creatine and taurine were signiciantly increased, but isoleucine, leucine, glutamine, choline and betaine/TMAO were decreased in the model rats. Conclusion ^1H NMRspectroscopy and pattern recognition can give the metabolic characteristics of liver tissue extracts from fructose-fed rats, and also provide a reliable metabolic basis for mechanism research of fatty generation of liver on the molecular level.