| 淋病奈瑟菌共同抗原表位在淋病快速检测中的临床应用价值 |
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| 引用本文： | 胡春华,李玲,陈雷,郑新华,李刚,刘道忠,裴娇,廖芳. 淋病奈瑟菌共同抗原表位在淋病快速检测中的临床应用价值[J]. 华中科技大学学报(医学版), 2011, 40(2): 192-195. DOI: 10.3870/j.issn.1672-0741.2011.02.015 |
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| 作者姓名： | 胡春华 李玲 陈雷 郑新华 李刚 刘道忠 裴娇 廖芳 |
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| 作者单位： | 1. 武汉市皮肤病防治所,武汉,430032
2. 华中科技大学同济医学院基础医学院病原生物学系,武汉,430030 |
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| 基金项目： | 湖北省科技攻关计划资助项目 |
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| 摘 要： | 目的 研究淋病奈瑟菌共同抗原表位在淋病快速检测中的临床应用价值.方法 根据各型淋病奈瑟菌外膜蛋白PIB上共同抗原表位的氨基酸序列合成一段多肽(WSWLF),并将其与钥孔血蓝蛋白(KLH)偶联构成完全抗原WSWLF-KLH,免疫动物,收集抗血清,ELISA和Western blot检测WSWLF-KLH免疫原性和特异性,...
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| 关 键 词： | 淋病奈瑟菌 共同抗原表位 免疫原性 检测试剂 |
Applied Value of Common Immunodominant Epitope of Neisseria Gonococcus in Rapid Detection of Gonorrhea |
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| Hu Chunhua,Li Ling,Chen Lei et al Wuhan Institute of Dermatology , Venereology,Wuhan ,China. Applied Value of Common Immunodominant Epitope of Neisseria Gonococcus in Rapid Detection of Gonorrhea[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 2011, 40(2): 192-195. DOI: 10.3870/j.issn.1672-0741.2011.02.015 |
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| Authors: | Hu Chunhua Li Ling Chen Lei et al Wuhan Institute of Dermatology Venereology Wuhan China |
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| Affiliation: | Hu Chunhua,Li Ling,Chen Lei et al Wuhan Institute of Dermatology and Venereology,Wuhan 430032,China |
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| Abstract: | Objective To study the applied value of common epitope of gonococcus in rapid detection of gonorrhea.Methods A polypeptide(WSWLF)was synthesized according to the amino acid sequence of common epitope in the outer membrane protein PIB of different types of gonococci.The polypeptide was coupled with Keyhole limpet hemocyanin(KLH)to construct complete antigen WSWLF-KLH to immune animals and the antiserum was harvested.ELISA and Western blot were used to detect the immunogenicity and specificity.The antiserum w... |
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| Keywords: | gonococcus common epitope immunogenicity detection reagent |
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