| 顺铂诱导骨肉瘤细胞自噬及其与凋亡关系的体外研究 |
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| 引用本文： | 张志才,邵增务,熊蠡茗,车彪,邓超,杨述华.顺铂诱导骨肉瘤细胞自噬及其与凋亡关系的体外研究[J].华中科技大学学报(医学版),2011,40(2):178-182. |
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| 作者姓名： | 张志才 邵增务 熊蠡茗 车彪 邓超 杨述华 |
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| 作者单位： | 华中科技大学同济医学院附属协和医院骨科,武汉,430022 |
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| 摘 要： | 目的研究顺铂(DDP)诱导的骨肉瘤细胞自噬性活化,并探讨自噬与凋亡的关系。方法利用免疫荧光染色和电镜观察DDP对骨肉瘤细胞自噬的影响;应用3-甲基腺嘌呤(3MA)下调自噬,采用MTT比色法,测定DDP对骨肉瘤细胞增殖抑制率的影响;MDC荧光染色和流式细胞术定量检测骨肉瘤细胞自噬的表达;应用流式细胞仪检测骨肉瘤细胞的凋亡情况。结果 DDP给药后可见LC3-Ⅱ绿色荧光呈点状散在分布于细胞膜附近。电镜下观察到DDP给药后骨肉瘤细胞内自噬体的形成。DDP能诱导自噬和凋亡的发生。3MA能抑制自噬的发生,对凋亡无明显影响。与单独DDP作用相比,3MA提前给药细胞的凋亡率增加,其细胞增殖抑制率也增高;3MA迟后给药细胞的凋亡率略有下降,其细胞增殖抑制率也下降。结论 DDP能诱导MG63细胞发生自噬性活化。DDP作用早期,自噬能延缓凋亡的发生,发挥保护细胞的作用;在DDP作用后期,自噬则作为另一种死亡形式出现,和凋亡一起促进细胞死亡。
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| 关 键 词： | 骨肉瘤 自噬 细胞凋亡 顺铂 |
In vitro Study on the Relationship between Autophagy and Apoptosis Induced by Cisplatin in Osteosarcoma |
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| Zhang Zhicai,Shao Zengwu,Xiong Liming et al.In vitro Study on the Relationship between Autophagy and Apoptosis Induced by Cisplatin in Osteosarcoma[J].Journal of Huazhong University of Science and Technology(Health Sciences),2011,40(2):178-182. |
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| Authors: | Zhang Zhicai Shao Zengwu Xiong Liming |
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| Institution: | Zhang Zhicai,Shao Zengwu,Xiong Liming et al Department of Orthopedics,Union Hospital,Tongji Medical College,Huazhong University ofScience and Technology,Wuhan 430022,China |
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| Abstract: | Objective To explore the relationship between autophagy and apoptosis induced by cisplatin(DDP).Methods The effects of autophagy in osteosarcoma were investigated by immunofluorescence staining and electron microscope.Through down-regulation of autophagy with 3-methyladenine(3MA),the proliferation inhibition ratio of MG63 cells with DDP was measured by using MTT assay.The expression of autophagy was examined by MDC staining and flow cytometry.The apoptosis of MG63 cells was detected by flow cytometry.Result... |
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| Keywords: | osteosarcoma autophagy apoptosis cisplatin |
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