一氧化氮合酶在人子宫内膜和蜕膜的表达

为探讨一氧化氮(NO)在子宫内膜功能性活动中所发挥的作用,应用免疫组织化学技术检测人增生期、分泌期子宫内膜及早孕期蜕膜内皮型一氧化氮合酶(eNOS)和诱导型一氧化氮合酶(iNOS)的表达模式.结果发现①增生期仅内膜血管内皮细胞表达eNOS,iNOS不表达于内膜的任何组份;②分泌期两种NOS都表达于子宫内膜的腺上皮和腔上皮.子宫内膜间质细胞未检测到NOS.③蜕膜组织蜕膜组织腺上皮和腔上皮eNOS和iNOS的表达明显增强,并且蜕膜间质细胞表达iNOS.分泌期子宫内膜和蜕膜组织表达eNOS和iNOS增加,表明此时NO的产量增多,而NO又可能通过其扩张血管、松弛平滑肌和促凋亡的作用参与胚胎着床的发生和月经的形成.

Expression of Nitric Oxide Synthase Isoforms in Human Endometrium and Decidua

To evaluate the role of nitric oxide in local function of endometrium, the pattern of expression of endothelial and inducible nitric oxide synthases in the human endometrium at proliferative and secretory phases and decidua was studied by using immunocytochemistry Results showed: (1) At the proliferative phase, the eNOS immunostaining was confined to vascular endothelial, whereas the iNOS was not detected in any composition of endometrium (2) At the secretory phase, surface epithelium and grandular epithelium showed positive staining for both eNOS and iNOS The stroma remained uniformly negative throughout the menstrual cycle (3) In the decidua, the expression of both isoforms was increased, while moderate iNOS immunoreactivity was observed in decidualized stromal cells It was demonstrated that the expression of eNOS and iNOS was elevated at the secretory phase and in decidua, indicating the increased production of NO at these phases The increasing of NO might take part in implantation through dilating the vessels and relaxing the smooth muscles and in menstration through promoting apoptosis