结肠癌患者血清IGF-1水平及癌组织VEGF表达的临床意义

目的 检测结肠癌患者血清中胰岛素样生长因子-1（IGF-1）的含量及癌组织中血管内皮生长因子（VEGF）的表达，并探讨其在结肠癌中的临床意义。方法 应用双位点免疫放射测定法（IRMA）对不同Dukes分期的56例结肠癌患者术前术后血清IGF-1进行检测。应用SP免疫组化法检测各组结肠癌组织VEGF的表达，14例健康体检者设为正常对照。结果结肠癌患者术前血清IGF-1水平明显高于术后水平及正常对照，结肠癌组织VEGF表达率较正常对照组高，差异均有显著性意义（均P〈0．05），不同病理分期的结肠癌血清IGF-1水平与癌组织VEGF阳性表达间差异有极显著性意义（P〈0．01）。结论 结肠癌患者血清IGF-1水平与结肠癌Dukes分期呈正相关，与癌组织VEGF表达密切相关，提示IGF-1和VEGF可能参与了结肠癌的发生发展过程，有望作为结肠癌诊治的重要检测指标。

Clinical Significance of Serum IGF-1 Level and Tissue VEGF Expression in Colon Cancer Patients

Objective To detect the serum insulin-like growth factor-1(IGF-1) level and tissue VEGF expression in patients with colon cancer and investigate its clinical significance.Methods The serum IGF-1 level was determined by immunoradiometric assay(IRMA) and the expression of tissue VEGF was detected by SP immunohistochemical assay in 56 pre-and post-operative cases of colon cancer(based on Dukes staging).Fourteen normal subjects were taken as control group.Results The serum IGF-1 level before operation was higher than that in control group and postoperation.VEGF expression rate was higher in tumor tissue than normal tissue,and the difference was significant(P<0.05).In patients with different pathological stages the serum IGF-1 level and tumor VEGF expression was statistically significant(P<0.01).Conclusion The serum IGF-1 level and tumor VEGF expression in different stages of colon cancer was proportional to the severity of disease,so the serum IGF-1 level and VEGF expression may be an indicator of cancer occurrence and progression.Therefore,they may become an important diagnostic marker in colon cancer.