肺吸虫抗原与日本血吸虫病兔血清的交叉反应

肺吸虫成虫粗抗原与日本血吸虫病兔血清可出现交叉反应,煮沸后的成虫粗抗原与血吸虫病兔血清也存在交叉反应。成虫粗抗原经Sephadex G-200过柱后得到两个蛋白峰,环状沉淀试验、对流免疫电泳和间接血凝试验证实,第1个蛋白峰与血吸虫病兔血清存在交叉反应,第2个蛋白峰无交叉反应。成虫粗抗原、煮沸的成虫粗抗原和过柱后得到的两个蛋白峰,分别经圆盘电泳后作过碘酸雪夫(PAS)染色,证实第2个蛋白峰不含糖蛋白。由此推断,肺吸虫成虫粗抗原与抗血吸虫血清发生交叉反应的成分是一类热稳定的糖蛋白。

The Cross Reaction Between Antigen of Paragonimus and Serum of Rabbit Infected with Schistosoma Japonicum

A cross reaction was observed between the crude antigen of Paragonimus adult worm (PW) and the serum of the rabbit infected with Schistosoma japonicum. The cross reaction also existed between the same antigen which had been boiled (PWB) and the same serum stated above. When the crude antigen of Paragonimus passed through the gel filtration on sephadex G-200, it yielded 2 peaks (P1, P2). The component of the cross reactive antigen in P2 was proved neither by ring precipitin test, counter immunoelectrophoresis nor by indirect haemagglutinayion test. No glycoprotein in P2 was demonstrated when the crude antigen and the boiled crude anti-gen as well as P1 and P2 were analysed by disc electrophoresis and stained by periodic acid-schiff (PAS) respectively. These results suggest that the cross reactive antigen substance in the crude antigen of Par-agonimus adult worm should be a kind of heat-stable glycoprotein.