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| 人组织型激肽释放酶基因对2型糖尿病大鼠AMP激活的蛋白激酶活性的影响 | |
| 引用本文： | 徐西振,凃玲,赵刚,袁刚,汪道文.人组织型激肽释放酶基因对2型糖尿病大鼠AMP激活的蛋白激酶活性的影响[J].华中科技大学学报(医学版),2008,37(3). |
| 作者姓名： | 徐西振 凃玲 赵刚 袁刚 汪道文 |
| 作者单位： | 1. 华中科技大学同济医学院附属同济医院心血管内科,武汉,430030 2. 华中科技大学同济医学院附属同济医院综合医疗科,武汉,430030 3. 华中科技大学同济医学院附属同济医院内分泌科,武汉,430030 |
| 摘 要： | 目的 研究人组织型激肽释放酶(human tissue kallikrein, HK)基因对链脲佐菌素(streptozotocin,STZ)诱导的2型糖尿病(diabete mellitus, DM)大鼠AMP激活的蛋白激酶(AMP-activated protein kinase, AMPK)活性的影响.方法 48只雄性Wistar大鼠随机分为普通饲料组,即正常对照组(n=16),和高脂高糖饲料组(n=32),高脂高糖饲料组大鼠以小剂量STZ加高脂高糖饮食诱导2型DM,再将其随机分为两组,分别经尾静脉导入重组腺相关病毒(recombinant adeno-associated viruses, rAAV)介导的HK基因(HK组)以及报告基因beta-半乳糖苷酶(beta-galactosidase, Lacz)(Lacz组),观察12周后处死实验动物,留取心脏、肝脏、肾脏等组织.Western blot法检测HK在各脏器的表达以及AMPK的表达及其磷酸化水平(phospho-AMPK).结果 rAAV介导的HK可在2型DM大鼠的肝脏、肾脏、心脏组织表达,且HK组各脏器中HK蛋白表达显著高于Lacz组(均P<0.05).Western blot检测发现,在肝脏、肾脏、心脏、骨骼肌、脂肪等脏器中,HK组和Lacz组AMPK的表达与正常对照组比较,差异无显著性意义(均P>0.05),而HK组phospho-AMPK较正常对照组显著上调(P<0.05),Lacz组phospho-AMPK与正常对照组比较,差异无显著性意义(P>0.05).结论 rAAV载体介导的HK基因能够激活2型DM大鼠主要器官AMPK的表达. |
| 关 键 词： | 人组织型激肽释放酶 AMP激活的蛋白激酶 糖尿病 人组织型 激肽释放酶 基因对 糖尿病大鼠 激活 蛋白表达 激酶活性 影响 Diabetic Rats Protein Kinase Activities Gene Tissue Kallikrein Human 器官 载体介导 意义 差异 比较 脂肪 |
Effects of Human Tissue Kallikrein Gene on Activities of AMP-activated Protein Kinase in Diabetic Rats |
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| Xu Xizhen,Tu Ling,Zhao Gang et al.Effects of Human Tissue Kallikrein Gene on Activities of AMP-activated Protein Kinase in Diabetic Rats[J].Journal of Huazhong University of Science and Technology(Health Sciences),2008,37(3). | |
| Authors: | Xu Xizhen Tu Ling Zhao Gang |
| Institution: | Xu Xizhen1,Tu Ling2,Zhao Gang2 et al1Department of Cardiology,2Department of Integrative Medicine,Tongji Hospital,Tongji Medical College,Huazhong University of Science , Technology,Wuhan 430030 |
| Abstract: | Objective To investigate the effects of human tissue kallikrein (HK) gene on activities of AMP-activated protein kinase in streptozotocin (STZ)-induced diabetic rats. Methods Forty-eight male Wistar rats were divided into control and experimental groups randomly. Wistar rats in experimental group were injected with low dose STZ (25 mg/kg) and fed with diets enriched in both glucose and fat to establish type 2 diabetic models, then subdivided into 2 subgroups randomly (each having 8 diabetic rats). Recombina... |
| Keywords: | human tissue kallikrein AMP-activated protein kinase diabete mellitus |
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