根尖周肉芽肿内厌氧菌的间接免疫荧光检测研究

本文收集36例很尖周肉芽肿标本,用兔抗厌氧消化链球菌、齿龈类杆菌、中间型类杆菌和小韦永球菌的多克隆抗血清,对其进行间接免疫荧光组织化学检测;同时对照HE染色和Gram染色观察。结果36例标本中有20例(55.6％)检出厌氧消化链球菌抗原,有22例(61.1％)检出齿龈类杆菌和中间型类杆菌抗原,而小韦永球菌仅发现2例;上述抗原定位于根尖周肉芽肿的渗出区、坏死区及根尖面,并与巨噬细胞有关。

Detection of Anaerobia in Human Periapical Granulomas Using Indirect Immunofluorescence

Serial eryostat sections of 36 odontogenic periapical granulomas of human tooth were prepared for demonstration of anaerobic bacteria by using indirect immuunofluo-rescencetechnique. The rabbit were against Peptostreptococaus anerobius(P. a), Becteroides gingivalis (B.g) , Becteroides intermedius (B. i) and Veillonella parvula (V.p) were used for identification of the bacteroides in this study. The sections of positive immunofluorescence were compared with the HE-stained and Gram-stained sections of the same sample. The results were as follows: There were 20 (55. 6%) antigens of P. a, 22 (61.1 %) antigens of B. g and antigens of B. i, and only 2 (5. 6 %) antigens of V. p have been found in 36 samples. The anaerobic bacteria with their antigens as mentioned above were found in the exudative zone, necro'tic zone and granulomas adjacent to the root-apex, and were also related to macrophage.