| 能合成和分泌GABA的永生化神经前体细胞的构建 |
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| 引用本文： | 笱玉兰,朱遂强,陈国华,张继龙,罗利俊,阮旭中.能合成和分泌GABA的永生化神经前体细胞的构建[J].华中科技大学学报(医学版),2007,36(6):721-724,846. |
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| 作者姓名： | 笱玉兰 朱遂强 陈国华 张继龙 罗利俊 阮旭中 |
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| 作者单位： | 1. 武汉市第一医院神经内科,武汉,430022
2. 华中科技大学同济医学院附属同济医院神经内科,武汉,430030 |
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| 摘 要： | 目的构建能合成和分泌γ-氨基丁酸（γ-aminobutyric acid，GABA）且可以无限增殖的永生化神经前体细胞。方法在永生化的神经前体细胞中转人含有GABA合成限速酶谷氨酸脱羧酶65亚型（glutamate decarboxylase 65，GAD65）的基因，用免疫组织化学、Western blot、毛细管电泳等方法观察GABA的合成和分泌。结果与对照组相比，INPCs—GAD65的含量明显增加。合成和释放的GABA显著增加，并能记录到GABA电流。结论成功构建了能合成和分泌GABA的永生化的神经前体细胞。
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| 关 键 词： | 谷氨酸脱羧酶65亚型 γ-氨基丁酸 神经前体细胞 |
| 收稿时间： | 2007-02-06 |
| 修稿时间： | 2007年2月6日 |
Construction of Genetically Immortalized Neural Progenitor Cells Synthesizing and Secreting GABA in Rats |
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| Gou Yulan , Zhu Suiqiang ,Chen Guohuaet al.Construction of Genetically Immortalized Neural Progenitor Cells Synthesizing and Secreting GABA in Rats[J].Journal of Huazhong University of Science and Technology(Health Sciences),2007,36(6):721-724,846. |
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| Authors: | Gou Yulan Zhu Suiqiang Chen Guohua |
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| Abstract: | Objective To construct genetically engineered cell lines to synthesize and release GABA by immortalized neural progenitor cells(INPCs) in rats.Methods The INPCs in rats were transfected with the GABA-synthesizing enzyme GAD(65) cDNA(INPCs-GAD65).The synthesis and secretion of GABA was observed by capillary electropherograms and the expression of GAD65 detected by immunohisochemistry and Western blot,respectively.GABA current was recorded in INPCs using whole-cell voltage clamp technique.Results In INPCs-GAD65,the contents of GAD65 were significantly increased as compared with control group,GABA were synthesized and secreted,and the GABA current was recorded.Conclusion The INPCs-GAD65 synthesizing and secreting GABA were successfully constructed. |
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