| 静电纺丝聚乳酸/聚己内酯共混纤维支架与兔脂肪源干细胞的体外生物相容性研究 |
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| 引用本文： | 陈亮,彭鄂军,吴柏霖,曾晓勇,庄乾元,叶章群.静电纺丝聚乳酸/聚己内酯共混纤维支架与兔脂肪源干细胞的体外生物相容性研究[J].华中科技大学学报(医学版),2011,40(5):513-516. |
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| 作者姓名： | 陈亮 彭鄂军 吴柏霖 曾晓勇 庄乾元 叶章群 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院泌尿外科,武汉,430030 |
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| 基金项目： | 国家自然科学基金，教育部留学回国人员科研启动基金，华中科技大学科学研究基金 |
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| 摘 要： | 目的利用兔脂肪源干细胞评价静电纺丝聚乳酸/聚己内酯共混纤维支架的生物相容性。方法脂肪源干细胞取自新西兰白兔皮下脂肪组织,经分离、培养后接种到待检支架上,使用倒置相差显微镜和扫描电镜观察细胞在支架上的生长形态;MTT法比较支架上培养和常规培养条件下的脂肪源干细胞生长曲线与倍增时间。结果脂肪源干细胞在纤维支架上生长良好,生长曲线与常规培养时基本一致,两组间细胞倍增时间差异无统计学意义(P>0.05)。结论脂肪源干细胞能够在静电纺丝聚乳酸/聚己内酯共混纤维支架材料上正常生长增殖,该支架材料生物相容性好。
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| 关 键 词： | 支架 生物相容性 脂肪源干细胞 静电纺丝 聚乳酸 聚己内酯 |
Biocompatibility of Electrospun PLA/PCL Blend Fibrous Scaffolds with Rabbit Adipose-Derived Stem Cells In Vitro |
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| Chen Liang,Peng Ejun,Wu Bolin et al.Biocompatibility of Electrospun PLA/PCL Blend Fibrous Scaffolds with Rabbit Adipose-Derived Stem Cells In Vitro[J].Journal of Huazhong University of Science and Technology(Health Sciences),2011,40(5):513-516. |
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| Authors: | Chen Liang Peng Ejun Wu Bolin |
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| Institution: | Chen Liang,Peng Ejun,Wu Bolin et al Department of Urology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China |
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| Abstract: | Objective To evaluate the biocompatibility of electrospun PLA/PCL blend fibrous scaffold with rabbit adipose-derived stem cells(ASCs). Methods ASCs were obtained from subcutaneous adipose tissues of a New Zealand white rabbit.After isolation and culture,ASCs were seeded on the target scaffolds.The cellular morphology was observed under the inverted phase contrast microscopy(IPCM)and scanning electron microscopy(SEM).The growth curve and doubling time of ASCs in scaffolds and in conventional culture were com... |
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| Keywords: | scaffold biocompatibility adipose-derived stem cells electrospinning poly(lactic acid) poly(ε-caprolactone) |
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