| IL-2基因转染细胞激活非特异性免疫杀伤细胞的抗肿瘤作用 |
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| 引用本文： | 张慧,范曲,张桂梅,李东,冯作化.IL-2基因转染细胞激活非特异性免疫杀伤细胞的抗肿瘤作用[J].华中科技大学学报(医学版),1997(3). |
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| 作者姓名： | 张慧 范曲 张桂梅 李东 冯作化 |
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| 基金项目： | 国家自然科学基金!39470332 |
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| 摘 要： | 以NIH3T3细胞作为载体细胞，用基因转染的方法建立了持续分泌白细胞介素2（IL－2）的细胞株。在体外此细胞上清可以增强小鼠脾细胞和腹腔巨噬细胞对黑色素瘤B16细胞的杀伤作用（与对照组比较，均为P＜0．01）。体内注射基因转染细胞则可以抑制黑色素瘤细胞在体内的生长（与对照组比较，均为P＜0．01）。结果表明，分泌IL-2的成纤维细胞可以通过激活非特异性免疫杀伤细胞而达到抗肿瘤的作用，是一种有较大潜力的肿瘤生物治疗的新途径。
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| 关 键 词： | 白细胞介素2 肿瘤 基因疗法 成纤维细胞 |
The Enhancement of Anti-tumor Function of Nonspecific Immune Cells by IL-2 Gene-transfected Cells |
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| Zhang Hui, Fan Qu, Zhang Guimei et al.The Enhancement of Anti-tumor Function of Nonspecific Immune Cells by IL-2 Gene-transfected Cells[J].Journal of Huazhong University of Science and Technology(Health Sciences),1997(3). |
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| Authors: | Zhang Hui Fan Qu Zhang Guimei |
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| Abstract: | NIH3T3 cells transfected with interleukin 2 (IL-2) gene could continuously secrete IL-2. The supernatant of trans fected celis could enhance the cytotoxic effects of murine splenocytes and peritoneal macrophages on melanoma B16 cells (compared with control group, P < 0.01). The proliferation of melanoma cells in vivo was inhibited by administration of gene-trans fected N1H3T3 cells. The results suggest that IL-2-secreting NIH3T3 cells have the anti- tumor function by enhancing the function of nonspecific immune killer cells. This is a potentially useful new approach in tumor therapy. |
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| Keywords: | interleukin 2 tumor gene therapy fibroblast |
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