建立转bcl-xL基因小鼠的研究

目的 建立转bcl—xL基因小鼠，继而传代建系，用于缺血性脑血管疾病的研究。方法 采用显微注射法，将人bcl—xL基因注入民明小白鼠受精卵获得子代鼠，然后作PCR、Southern—blot、mRNA、Western—blot检测以获得阳性鼠。结果 实验中注射受精卵1654枚，移植卵数1448枚，受体鼠49只，怀孕鼠7只，子代鼠13只，整合4只并均有表达。注射受精卵的存活率87．54％，受精卵的总存活率0．78％，受体鼠的怀孕率14．28％，整合效率30．77％，总有效率为0．24％。结论 获得了转bcl—xL基因小鼠，但转基因的效率较低，表达不稳定。

Establishment of bcl-xL Transgenic Mice

Objective To develop bcl xL transgenic mouse and produce its lineages for studying ischemic cerebrovascular diseases. Methods Bcl xL gene was introduced into mice by microinjection. The presence of bcl xL and its expression were confirmed by PCR, Southern blot, mRNA inspection, Western blot in the founders. Results In our experiments, bcl xL gene was injected into pronucleus of 1654 zygotes and 1448 injected eggs were implanted into 49 foster mothers, 7 of which were pregnant and bore 13 offsprings. Among the babies, 4 offsprings were integrated with the bcl xL gene with the detectable expression. Totally, implanted zygotes' survival rate was 87 54 %, zygotes' total survival rate was 0 78 %, recipient mouse pregnance rate was 14 28 %, integration rate was 30 77 %, expression rate was 100 % and, total efficiency was 0 24 % respectively. Conclusion Bcl xL transgenic mice ( n =4) were obtained, but the transgenic efficiency was very low and the expression was unstable.