| 缝隙连接对局灶性脑缺血后海马迟发性神经元死亡及神经行为学的影响 |
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| 引用本文： | 易陈菊,徐沙贝,唐颖馨,张强,骆翔,王伟.缝隙连接对局灶性脑缺血后海马迟发性神经元死亡及神经行为学的影响[J].华中科技大学学报(医学版),2008,37(6). |
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| 作者姓名： | 易陈菊 徐沙贝 唐颖馨 张强 骆翔 王伟 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院神经内科,武汉,430030 |
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| 基金项目： | 国家自然科学基金，国家杰出青年科学基金，湖北省自然科学基金 |
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| 摘 要： | 目的探讨阻断缝隙连接对大鼠局灶性脑缺血后海马迟发性神经元死亡（DND）及神经行为学的影响。方法术前2h左侧脑室注射缝隙连接阻断剂甘珀酸（CBX），对照组左侧脑室注射生理盐水，颈内动脉插线法制备大鼠大脑中动脉缺血-再灌注模型。术后72h采用尼氏染色检测海马迟发性神经元死亡，术后24h和72h进行神经行为学评分．数据进行统计学分析，观察阻断缝隙连接对大鼠局灶性脑缺血72h后海马迟发性神经元死亡及神经行为学的影响。结果不用CBX预处理，大脑中动脉缺血模型72h后有45％的动物出现海马DND，用CBX预处理后．DND发生率降到30％．较对照组明显降低（P〈0．01）。CBX干预组的行为学评分明显比对照组低（P〈0．01）。结论阻断缝隙连接可以减少局灶性脑缺血后海马迟发件神经元死亡的发生率，改善局灶性脑缺血术后动物行为学表现。
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| 关 键 词： | 缝隙连接 局灶性脑缺血 迟发性神经元死亡 神经行为学评分 甘珀酸 |
Effect of Gap Junction on Delayed Neuronal Death in Rat Hippocampus after Middle Cerebral Artery Occlusion and the Neurological Scores |
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| Yi Chenju,Xu Shabei,Tang Yingxin et al.Effect of Gap Junction on Delayed Neuronal Death in Rat Hippocampus after Middle Cerebral Artery Occlusion and the Neurological Scores[J].Journal of Huazhong University of Science and Technology(Health Sciences),2008,37(6). |
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| Authors: | Yi Chenju Xu Shabei Tang Yingxin |
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| Institution: | Yi Chenju,Xu Shabei,Tang Yingxin et al Department of Neurology,Tongji Hospital,Tongji Medical College,Huazhong University of Science , Technology,Wuhan 430030 |
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| Abstract: | Objective To explore the effect of blocking gap junction communication after focal cerebral ischemia on delayed neuronal death in ipsilateral hippocampus and the neurological scores. Methods A rat model of middle cerebral artery occlusion (MCAO) was made by the intraluminal occlusion technique. Carbenoxolone,a gap junction blocker,was used to block gap junction communication,and the effect of blocking gap junction communication after focal cerebral ischemia on delayed neuronal death in the ipsilateral hippo... |
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| Keywords: | gap junction focal cerebral ischemia delayed neuronal death neurological score carbenoxolone |
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