轮状病毒酶试验在婴幼儿腹泻病原学诊断中的应用

<正> 轮状病毒是引起婴幼儿秋季腹泻的主要病原,如能早期作出病原学诊断,对于指导治疗和判断预后具有十分重要的意义。为此我们于1982年10～11月收集了77例婴幼儿腹泻大便标本,应用具有高度特异性的轮状病毒酶试验

APPLICATION OF ROTAZYME TEST IN PATHOGENIC DIAGNOSIS OF INFANTILE DIARRHEA

Rotavirus is the major cause of non bacterial gastroenteritis in infant. Since the human rotavirus cannot be grown readily in tissue culture, the diagnosis of its presence in stool must be done by electronmicroscopy in former times. The rotazyme test is an immunoassay. It can detect rotavirus antigen rapidly in human fecal material. This paper reports the results of an experiment of 77 infantile diarrhea cases' fecal specimens which were examined both by rotazyme test and electronmicroscopy with the results of 55 specimens proved to he positive and 22 ones negative by rotazyme test. Of the. specimens examined by electronmicroscopy. the positive specimens were 50 and the negative ones 27. Samples proved to be positive by rotazyme test and negative by electronmicroscopy could be confirmed by inhibition test. These results reveals that the rotnzyme test is a simple and high-speed method in the stead of electronmicroscopy in examination of the infantile diarrhea fecal specimens.