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盐酸阿比朵尔抗呼吸道合胞病毒机制初探
引用本文:何静,杨占秋,石丽桥,刘媛媛,肖红.盐酸阿比朵尔抗呼吸道合胞病毒机制初探[J].武汉大学学报(医学版),2006,27(1):69-71.
作者姓名:何静  杨占秋  石丽桥  刘媛媛  肖红
作者单位:武汉大学医学院病毒学研究所/病毒学国家重点实验室,武汉,430071
摘    要:目的:明确盐酸阿比朵尔抗吸道合胞病毒(RSV)Long株的活性并探讨其抗病毒机制。方法:通过细胞病变效应(CPE)、噻唑蓝(MTT)比色法检测细胞活性,观察盐酸阿比朵尔抗RSV的作用。结果:盐酸阿比朵尔的半数毒性浓度(TD50)为85.39 mg/L,当该药浓度为25 mg/L时,药物抗病毒生物合成组、药物直接作用组、药物抗吸附4 h组、药物抗病毒吸附8 h组的病毒抑制率分别能达到81.61%,79.57%,40.39%和78.60%。4组相应的药物半数有效浓度(ED50)为11.52,8.74,25.36和10.40 mg/L。结论:盐酸阿比朵尔在体外能明显抑制RSV引起的CPE效应,病毒抑制率随药物浓度的增加而增高,盐酸阿比朵尔能通过多种途径发挥抗RSV的作用。

关 键 词:盐酸阿比朵尔  呼吸道合胞病毒  抗病毒作用
文章编号:1671-8852(2006)01-0069-03
收稿时间:2005-03-11
修稿时间:2005年3月11日

Anti-viral Activity of Arbidol Hydrochloride Against Respiratory Syncytial Virus in Vitro
HE Jing,YANG Zhanqiu,SHI Liqiao,LIU Yuanyuan,XIAO Hong.Anti-viral Activity of Arbidol Hydrochloride Against Respiratory Syncytial Virus in Vitro[J].Medical Journal of Wuhan University,2006,27(1):69-71.
Authors:HE Jing  YANG Zhanqiu  SHI Liqiao  LIU Yuanyuan  XIAO Hong
Abstract:Objective: To study the antiviral activity of Arbidol hydrochloride on respiratory syncytial virus(RSV) Long strain in vitro.Methods: Anti-RSV effects were investigated by observing cytopathic effect(CPE) with MTT colorimetric assay.Results: The median cell inhibitory(TD_(50)) of Arbidol was 85.39 mg/L and at its concentration of 25 mg/L, the viral inhibitory rates were up to 81.61%,(79.57%,) 40.39% and 78.60% respectively in antiviral biological synthesis group,directly-killing-virus group and interfering-in-viral-absorption groups(pretreated cells with Arbidol for four and eight hours respe-ctively).The corresponding median efficacious(EC_(50)) concentrations of Arbidol(against) RSV in the four groups were 11.52,(8.74,)(25.36) and 10.40 mg/L,respectively.(Conclusion:) Arbidol could significantly inhibit cytopathic effect(CPE) of RSV-infected HEp-2 cells and increase survival rates of RSV-infected cells in a dose dependent manner,and could be used as an effective drug for RSV infection.
Keywords:Arbidol Hydrochloride  Respiratory Syncytial Virus  Antiviral Effect
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