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炎症性肠病患者淋巴毒素α、白介素1受体拮抗剂基因多态性与肿瘤坏死因子α,可溶性白介素2受体以及白介素6产量的关系
引用本文:夏冰,JBACrusius,张贵水,郭海建,邓长生,SGWMeuwisen.炎症性肠病患者淋巴毒素α、白介素1受体拮抗剂基因多态性与肿瘤坏死因子α,可溶性白介素2受体以及白介素6产量的关系[J].武汉大学学报(医学版),1997(3).
作者姓名:夏冰  JBACrusius  张贵水  郭海建  邓长生  SGWMeuwisen
作者单位:荷兰阿姆斯特丹自由大学医院胃肠科
摘    要:测定中国炎症性肠病患者淋巴毒素α基因和白介素1受体拮抗剂基因多态性与肿瘤坏死因子α、可溶性白介素2受体以及白介素6产量的关系。22例炎症性肠病患者(20例溃疡性结肠炎,2例克隆病)和10例健康对照者参加研究。淋巴毒素α基因和白介素1受体拮抗剂基因片段由基因组DNAPCR扩增而来,采用ELISA法测定周围血单个核细胞肿瘤坏死因子α,可溶性白介素2受体以及白介素6产量。结果:炎症性肠病淋巴毒素α基因型1和2杂合子略高于正常对照组(11/12对1/10,P=0049);而且淋巴毒素α等位基因2与周围血单个核细胞诱生的高水平肿瘤坏死因子α产量有关。白介素1受体拮抗剂基因多态与炎症性肠病无显著性相关。认为:中国人群炎症性肠病患者淋巴毒素α基因可能对肿瘤坏死因子α产量起一定作用。

关 键 词:淋巴毒素类  白细胞介素1  有丝分裂因子.淋巴细胞.肿瘤  抑制素  受体.有丝分裂原  基因  多态现象  Crohn病  结肠炎.溃疡性

Relation of Polymorphisms of Lymphotoxin α and Interleukin 1 Receptor Antagonist Genes to Secretion of Tumour Necrosis Factor α, Soluble Interleukin 2 Receptor and Interleukin 6 in Chinese Patients with Inflammatory Bowel Disease
Abstract:? To determine the relation of lymphotoxin α and interleukin 1 receptor antagonist genes to the secretion of tumour necrosis factor α, soluble interleukin 2 receptor and interleukin 6 in Chinese patients with ulcerative colitis. Patients and Methods: Twenty_two patients with inflammatory bowel disease(20 ulcerative colitis and 2 Crohn's disease) and 10 healthy ontrols were studied. Lymphotoxin α gene and interleukin_1 receptor antagonist gene fragments were amplified from genomic DNA by PCR. Tumour necrosis factor α, soluble interleukin 2 receptor and interleukin 6 production from peripheral blood mononuclear cells were measured by ELISA’ s. Results: The genotype 1 and 2 of lymphotoxin α was slightly higher in inflammatory bowel disease than in the healthy control (11/22 vs 1/10, P=0.049) and allele 2 of lymphotoxin α was related to higher tumour necrosis factor α production from peripheral blood mononuclear cells on stimulation. There was no association between inflammatory bowel disease and interleukin 1 receptor antagonist gene polymorphism. conclusion: Lymphotoxin α gene may play a role in relation to secretion of tumour necrosis factor α in Chinese patients with inflammatory bowel disease.
Keywords:lymphotoxins  interleukin 1  mitogenic factors  lymphocyte  neoplasms  inhibin  receptors  mitogen  genes  polymorphism(genetics)  crohn disease  colitis  ulcerative
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