炎症性肠病患者淋巴毒素α、白介素1受体拮抗剂基因多态性与肿瘤坏死因子α,可溶性白介素2受体以及白介素6产量的关系 |
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引用本文: | 夏冰,JBACrusius,张贵水,郭海建,邓长生,SGWMeuwisen.炎症性肠病患者淋巴毒素α、白介素1受体拮抗剂基因多态性与肿瘤坏死因子α,可溶性白介素2受体以及白介素6产量的关系[J].武汉大学学报(医学版),1997(3). |
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作者姓名: | 夏冰 JBACrusius 张贵水 郭海建 邓长生 SGWMeuwisen |
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作者单位: | 荷兰阿姆斯特丹自由大学医院胃肠科 |
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摘 要: | 测定中国炎症性肠病患者淋巴毒素α基因和白介素1受体拮抗剂基因多态性与肿瘤坏死因子α、可溶性白介素2受体以及白介素6产量的关系。22例炎症性肠病患者(20例溃疡性结肠炎,2例克隆病)和10例健康对照者参加研究。淋巴毒素α基因和白介素1受体拮抗剂基因片段由基因组DNAPCR扩增而来,采用ELISA法测定周围血单个核细胞肿瘤坏死因子α,可溶性白介素2受体以及白介素6产量。结果:炎症性肠病淋巴毒素α基因型1和2杂合子略高于正常对照组(11/12对1/10,P=0049);而且淋巴毒素α等位基因2与周围血单个核细胞诱生的高水平肿瘤坏死因子α产量有关。白介素1受体拮抗剂基因多态与炎症性肠病无显著性相关。认为:中国人群炎症性肠病患者淋巴毒素α基因可能对肿瘤坏死因子α产量起一定作用。
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关 键 词: | 淋巴毒素类 白细胞介素1 有丝分裂因子.淋巴细胞.肿瘤 抑制素 受体.有丝分裂原 基因 多态现象 Crohn病 结肠炎.溃疡性 |
Relation of Polymorphisms of Lymphotoxin α and Interleukin 1 Receptor Antagonist Genes to Secretion of Tumour Necrosis Factor α, Soluble Interleukin 2 Receptor and Interleukin 6 in Chinese Patients with Inflammatory Bowel Disease |
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Abstract: | ? To determine the relation of lymphotoxin α and interleukin 1 receptor antagonist genes to the secretion of tumour necrosis factor α, soluble interleukin 2 receptor and interleukin 6 in Chinese patients with ulcerative colitis. Patients and Methods: Twenty_two patients with inflammatory bowel disease(20 ulcerative colitis and 2 Crohn's disease) and 10 healthy ontrols were studied. Lymphotoxin α gene and interleukin_1 receptor antagonist gene fragments were amplified from genomic DNA by PCR. Tumour necrosis factor α, soluble interleukin 2 receptor and interleukin 6 production from peripheral blood mononuclear cells were measured by ELISA’ s. Results: The genotype 1 and 2 of lymphotoxin α was slightly higher in inflammatory bowel disease than in the healthy control (11/22 vs 1/10, P=0.049) and allele 2 of lymphotoxin α was related to higher tumour necrosis factor α production from peripheral blood mononuclear cells on stimulation. There was no association between inflammatory bowel disease and interleukin 1 receptor antagonist gene polymorphism. conclusion: Lymphotoxin α gene may play a role in relation to secretion of tumour necrosis factor α in Chinese patients with inflammatory bowel disease. |
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Keywords: | lymphotoxins interleukin 1 mitogenic factors lymphocyte neoplasms inhibin receptors mitogen genes polymorphism(genetics) crohn disease colitis ulcerative |
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