PI3’K/AKT通路抑制剂对人胃癌细胞Caspase-9表达的影响

目的:检测PI3’K/Akt通路抑制剂对抗肿瘤药物诱导人胃癌细胞凋亡过程的影响。方法:将胃癌细胞系BGC-823细胞分为6组:空白对照组、渥曼青霉素组、足叶乙甙组、渥曼青霉素+足叶乙甙组、阿霉素组和渥曼青霉素+阿霉素组。按照上述分组用药物干预BGC-823细胞24 h,采用NaI法提取各组细胞的DNA,Western blot检测caspase-9表达变化及用琼脂糖凝胶电泳检测细胞凋亡情况。结果:①DNA琼脂糖凝胶电泳示空白对照组未见DNA梯状谱,其他5组均出现明显的DNA梯状谱;②Western blot结果提示阿霉素与足叶乙甙干预后,BGC-823的Caspase-9活性与未处理组相比增强,加用渥曼青霉素处理后,BGC-823的Caspase-9活性与未处理组相比明显增强,与单用阿霉素或足叶乙甙处理组相比有所增强。结论:PI3K/AKT通路抑制剂能促进胃癌细胞凋亡,增强抗肿瘤化疗药物的疗效。

Effect of PI3'K/AKt Inhibitor on the Expression of Caspase-9 in Human Gastric Cancer Cells

Objective:To investigate the effect of the inhibitor of PI3'K/Akt in inducing apoptosis of human gastric cancer cells interfered by chemotherapeutics.Methods:The gastric cancer cell line BGC-823 were divided into six groups as blank control,wortmannin,etoposide,wortmannin+etoposide,doxorubicin and wortmannin+doxorubicin group according to the different treatment. After 24 hours' treatment,DNA was extracted by NaI test and detected by agarose gel electrophoresis.The changes of caspase-9 expression were determined by Western blot analysis.Results:① DNA agrose gel electrophoresis showed that there were no apoptosis stripes in the control group,while pink stripes were found in the other groups;② Western blot showed that the activity of caspase-9 in the groups treated by doxorubicin or etoposide was higher than in the control group,and the activity of the groups treated by doxorubicin or etoposide plus wortmannin was higher than those treated by only one drug.Conclusion:The inhibitor of PI3K/AKt can up-regulate the apoptosis level of gastric cells and enhance the effect of chemotherapeutics.