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酶联免疫吸附试验(ELISA)测定风疹病毒抗体的探讨
引用本文:霍文哲,郭树春.酶联免疫吸附试验(ELISA)测定风疹病毒抗体的探讨[J].武汉大学学报(医学版),1984(3).
作者姓名:霍文哲  郭树春
作者单位:湖北医学院儿科研究所,湖北医学院儿科研究所
摘    要:本文介绍ELISA测定风疹病毒抗体的方法。用ELISA和HAI两种方法同时测定300份脐血。HAI效价≥1:8的274份阳性脐血中265份ELISA也是阳性。总的不符合率仅4.3%。比较来看,ELISA是一种简便、敏感和有效地检测风疹病毒抗体的方法。


PRELIMINARY STUDY ON APPLICATION OF ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TO DETECTION OF ANTIBODY AGAINST RUBELLA
Abstract:This paper describes the application of the ELISA method to detecting rubella antibody. This ELISA method was compared with haemagglutination inhibition in a study of 300 specimens of umbilical serum. Of the 274 specimens with HAI titres of 1:8 or higher, 265 were positive by ELISA. The total discrepancy rate between two tests was only 4.3%. The ELISA is a simple, sensitive and effective method for detecting rubella antibody.
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