高危HPV病毒第2代杂交捕获法检测应用于宫颈癌以及癌前病变检查

目的:探讨高危人类乳头瘤病毒(HPV)第二代杂交捕获实验(hc2)检测应用于宫颈癌以及癌前病变检查中的临床价值.方法:用hc2检测其子宫颈脱落细胞,以病理诊断作为金标准对检测结果进行分析和评价,对其特异度、敏感度、准确性、预测值等检测并分析.结果:hc2法检测高危型HPV的感染状况为无明确意义的非典型细胞的改变(ASC...

Application of Hybrid Capture 2 for High-Risk Human Papillomavirus Testing in Cervical Cancer and Premalignant Lesion

Objective:To explore the clinical value of hybrid capture 2(hc2) in detection of high risk HPV in cervical cancer and pre-cancerous cervical lesions.Methods:Cervix exfoliated cells were detected with the second generation of hybrid capture hc2 method,and the diagnostic results such as specificity,sensitivity,accuracy,and pedicted value were compared with that of pathological diagnosis respectively.Results:The infection rate of HPV assayed by hc2 was 43.8% in atypical squamous cell of undetermined signification(ASCUS),7.8% in ASCUS-H,9.7% low-grade squamous intraepithelial lesion(LSIL),and 28.7% in high-grade squamous intraepithelial lesion(HSIL) respectively according to TBS diagnosis and classification system.For high grade-squmous intraepithelial lesion≥(CINⅡ),the specificity,sensitivity,positive-predictive value and negative-predictive value by hc2 were 66.7%,93.4%,60.0%,and 95.0% respectively.In high virus load assay,there was significant difference between cervical cancer and CINⅠ(P<0.01),and between cervical cancer/CIN and NILM group(P<0.01),but there was no difference between CIN and cervical cancer.Conclusion:The high-risk HPV test with hc2 is still a good method in diagnosing cervical cancer and premalignant lesion.Examining the high-risk HPV viral load with hc2 is related to cervical cancer and premalignant lesions,but not related to the severity of cervical lesions.