抗氧化酶和脂质过氧化对大鼠睾丸扭转复位后生殖细胞凋亡的影响

目的 :研究大鼠睾丸扭转复位后抗氧化酶和脂质过氧化与生殖细胞凋亡的关系。方法 :成年健康SD雄性大鼠 (n =4 0 )随机分为实验组 (n =2 0 )和对照组 (n =2 0 )。建立单侧睾丸扭转 复位动物模型 (72 0° 2h)。采用TUNEL法检测生殖细胞凋亡并分别测定睾丸组织中超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSHPX)和丙二醛 (MDA)含量。结果 :实验组手术侧睾丸生殖细胞凋亡增多 ,MDA含量升高 ,SOD、GSHPX 活性降低 ,与对照组相比 ,两组差别具有极显著性意义 (P <0 .0 1)。结论 :睾丸扭转复位后生殖细胞凋亡增多与生殖细胞脂质过氧化作用增强和抗氧化酶活性下降有关

Effect of Antioxidant Enzyme and Lipid Peroxidation on Germ Cell Apoptosis in Testicular Torsion/Detorsion of Rats

Objective: To investigate the relationship between germ cell apoptosis and antioxidant enzyme and lipid peroxidation in experimental torsed/detorsed testes of the adult male rats. Methods: Healthy male Sprague Dawley rats(n=40)were divided into the torsion group(n=20 )and control group(n=20)randomly. Animals were submitted to unilateral 720°testicular torsion, then detorsion in two hours. Testes were examined for evidence of germ cell apoptosis by the TdT mediated dUTP biotin nick end labeling (TUNEL) technique and the level of superoxide dismutase(SOD), glutathione peroxidase (GSHP X) and malodialdehyde (MDA) by biochemical method. Results: Compared with that of the control groups,the apoptosis index of germ cell and average MDA level significantly increased in the torsed testes,but the activities of SOD and GSHP X both decreased remarkly(P<0.01). Conclusion: The increase of germ cell apoptosis is highly correlated with both lipid peroxidation enhancement and SOD and GSHP X activity decrease.