参附注射液对肾缺血再灌注损伤大鼠P38MAPK、TNF-α表达的影响

目的:探讨参附注射液(SF)对肾缺血再灌注损伤大鼠P38丝裂原活化蛋白激酶(MAPK)、肿瘤坏死因子(TNF-α)表达的影响。方法:采用切除右肾,无创动脉夹夹闭左肾肾蒂45 min,再灌注2 h制作在体肾缺血再灌注损伤模型。动物随机分为3组:预处理组、缺血再灌注组和对照组。免疫组织化学检测肾组织P38 MAPK蛋白含量,酶联免疫吸附实验(ELISA)检测肾组织TNF-α的表达。结果:预处理组与缺血再灌注组相比,肾脏病理损伤明显减轻,P38 MAPK、TNF-α蛋白含量显著降低(均为P<0.05)。结论:SF可能通过抑制P38 MAPK的表达,从而下调TNF-α的表达,发挥其肾脏保护作用。

Effect of Shenfu Injection on Expression of P38 MAPK and TNF-alpha after Renal Ischemia Reperfusion in Rats

Objective: To investigate the effect of Shenfu(SF) injection,a traditional Chinese medicine,on P38 mitogen activated protein kinase(MAPK)and tumor necrosis factor-alpha(TNF-α)after renal ischemia reperfusion(IR) in rats.Methods: Thirty-six male SD rats were divided into three groups randomly,with 12 rats in each group.Renal ischemia reperfusion injury was induced by left renal pedicle occlusion for 45 miniutes and followed by reperfusion with contralateral nephrectomy in rats.In IR+SF group,rats were pretreated with SF one hour before ischemia;IR+NS group was pretreated with 0.9% natrium chloride;in control group the left renal pedicle was isolated,however,occlusion of the pedicle was not performed.After two hours of reperfusion,kidneys were harvested,and P38 MAPK(assayed by imunohistochemistry) and TNF-α(assayed by ELISA)were determined.Results: In IR+SF group,the lesion of kidney were significantly alleviated;the activation of P38 MAPK and the expression level of TNF-α in kidney after IR were also inhibited when compared with those in the IR+NS group(both(P<0.01).)(Conclusion:) SF injection could downregulate the expression of TNF-α through inhibiting P38 MAPK pathway and play a role in a renal protective therapy.