基础研究cdc2基因在多种人类肿瘤细胞中的表达及意义

目的通过检测人表皮细胞Hacat和不同组织来源的6种肿瘤细胞的增殖能力以及cdc2基因在这些细胞中的表达情况,探讨cdc2基因与肿瘤发生、发展、增殖能力之间的关系。方法体外培养人表皮细胞Hacat和不同组织来源的6种肿瘤细胞(宫颈癌细胞Caski、神经胶质瘤细胞U87、肝癌细胞Hepg2、膀胱癌细胞T24、骨肉瘤细胞MG63、肺癌细胞A549)。采用逆转录聚合酶链式反应(RT-PCR)法和免疫印迹(Western blot)法测定细胞中cdc2在mRNA和蛋白质水平的表达情况;采用四甲基偶氮唑(MTT)法检测细胞增殖速度。结果 cdc2基因在Hacat中表达较低,在Caski、U87、Hepg2、T24、MG63和A549中表达较高。MTT法显示:Hepg2、U87、T24、A549、MG63增殖能力较强,Caski次之,Hacat最差。结论 cdc2基因可能参与了肿瘤的发生过程,并与肿瘤的增殖有关。

Expression and significance of cdc2 gene in cancer cells

Objective To investigate the expression of cdc2 gene in human epidermal cell Hacat and 6 human cancer cell lines,thus to study the correlation between the expression of cdc2 gene and the proliferation ability of cell.Methods RT-PCR and Western-blot assay were used to detect the expression levels of cdc2 mRNA and protein,respectively,in cell lines of Hacat,Caski,U87,Hepg2,T24,A549 and MG63.MTT assay was applied to assess the proliferation ability of cell.Results The expression of cdc2 was low in Hacat,while relative high expression was revealed in Caski,U87,Hepg2,T24,MG63 and A549.According to MTT assay,the proliferation ability of Hepg2,U87,Hepg2,T24,A549,MG63 was the highest,followed with Caski,and lowest in Hacat.Conclusion The cdc2 gene may involve in oncogenesis via participation in the cell proliferation.