结直肠癌组织中Tiam1、Fascin-1及HSPB1的表达及意义

目的探讨结直肠癌组织中Tiam1,Fascin-1及HSPB1的表达及意义。方法制作含100例结直肠癌组织的组织芯片,应用免疫组化SP法检测结直肠癌组织中Tiam1,Fascin-1及HSPB1的表达。结果组织芯片免疫组化染色后,形态可观测率为96%,并且背景清晰,对比鲜明。Tiam1表达阳性率为74%,Fascin-1表达阳性率为51%,HSPB1表达阳性率为68%,显著高于非肿瘤组织。Tiam1,Fascin-1及HSPB1表达与结直肠癌转移显著相关,伴发转移的结直肠癌组织Tiam1,Fascin-1及HSPB1阳性表达明显高于无转移者。通过相关性统计学分析,发现Tiam1与Fascin-1表达呈正相关(r=0.678,P<0.01),Tiam1与HSPB1表达呈正相关(r=0.650,P<0.01)。结论Tiam1,Fascin-1及HSPB1均与结直肠癌转移有关,Fascin-1和HSPB1的高表达可能与Tiam1的调控有关。

Expression and significance of Tiam1, Fascin-1 and HSPB1 in human colorectal cancer

Objective To investigate the expression of T lymphoma invasion and metastasis gene 1(Tiam1),Fascin-1 and heat shock 27kDa protein 1(HSPB1) in human colorectal cancer(CRC) and their significance.Methods A tissue microarray(TMA) containing 100 informative cases of CRC was constructed.Immunohistochemistry SP method was used to detect the expression of Tiam1,Fascin-1 and HSPB1 in TMA of CRC respectively.Results Ninety-six percents of dots on the TMA were observable morphologically with clear background and vivid contrast after immunohistochemistry.The positive rates of Tiam1,Fascin-1 and HSPB1 were 74%,51% and 68% respectively in TMA of colorectal cancer.Expression of Tiam1,Fascin-1 and HSPB1 protein in CRC with metastasis was higher than those in CRC without metastasis.This finding indicated that Tiam1,Fascin-1 and HSPB1 were positively correlated with metastasis of CRC.We next analyzed the correlation among Tiam1,Fascin-1 and HSPB1.Tiam1 expression was positively correlated with Fascin-1(r=0.678,P<0.01) and HSPB1(r=0.650,P<0.01) in colorectal cancer.Conclusion Tiam1,Fascin-1 and HSPB1 are related to metastasis of CRC.Overexpression of Fascin-1 and HSPB1 may relate to Tiam1 regulation.