参苓白术散对脾虚小鼠肠保护作用及其机制的研究

目的：研究参苓白术散对脾虚小鼠肠道菌群的调整及肠保护作用。方法：昆明小白鼠,分成5组：A组（模型组）;B组（正常对照组）;C组（脾虚自然恢复组）;D组（参苓白术散治疗组）;E组（丽珠肠乐治疗组）。检测结肠内容物和粪便标本的肠杆菌、肠球菌、双歧杆菌、类杆菌、乳酸杆菌数量。结肠组织光镜和电镜观察。结果：脾虚小鼠给予参苓白术散治疗后肠杆菌、肠球菌、双歧杆菌、类杆菌、乳酸杆菌数量逐渐恢复正常,肠壁肌层厚度增加,杯状细胞数量增多,肠粘膜微绒毛排列紊乱、线粒体肿胀显著改善。明显好于脾虚自然恢复组,与丽珠肠乐治疗组相比无明显差异。结论：参苓白术散对脾虚小鼠具有肠道菌群调整及促进损伤肠组织恢复的作用。

PROTECTION IN MOUSE MODEL WITH SPLEEN DEFICIENCY SYNDROME

Objective：This paper is to study the function of Shenlinbaizhu Powder to regulate intestional flora for the spleen deficiency mice and the intestines protection, nethods：KUNMING white mice were divided into five groups：group A （model group）, group B（ natural contrast group） ,group C（ Spleen deficiency natural cure group） ,group D（ Shenlinbaizhu Powder cure group） ,group E（ li zhu chang le cure group）. To detect colonic content and stool specimens contains Enterobacteriaceae, Enterococcus, Bifidobacteria, bacteroides, Lactobacillus. Observe colon organization with poticalmicroscope and electron microscope. Results： After one course of treatment with Shenlinbaizhu, the quantities of Enterobacteriaceae, Enterococcus, Bifldobacteria, bacteroides, lactobacilli return to normal intestine wall skin layer of thickness, cup form cell quantity increased, intestines mucous membrane little fine arrange order, mitochondria swell- ing improved notably. That was obviously better than Spleen Deficiency natural cure group and there had no remarkable difference from Li Zhu Chang Le cure group. Condusion：Shenlinbaizhu Powder has an effection on regulation on dysbateriorsis and promote recovery of colon damaged.