| 多种肿瘤组织中Nucleostemin基因表达的检测及意义 |
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| 引用本文： | 郑学芝,孙卫,徐秋玲,蔡子微.多种肿瘤组织中Nucleostemin基因表达的检测及意义[J].牡丹江医学院学报,2008,29(2):3-5. |
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| 作者姓名： | 郑学芝 孙卫 徐秋玲 蔡子微 |
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| 作者单位： | 牡丹江医学院生理教研室,黑龙江,牡丹江,157011 |
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| 基金项目： | 牡丹江医学院校科研和教改项目 |
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| 摘 要： | 目的:检测 Nucleostemin (NS)基因在多种肿瘤组织中的表达情况.方法:收集包括乳腺癌、卵巢癌、食管癌、胃癌等十几种肿瘤标本,试剂盒法提取组织总RNA,以GAPDH作为内参基因,用半定量RT-PCR方法检测NS基因在肿瘤组织中的表达情况.结果:所有肿瘤组织中皆有NS基因表达.结论:NS基因表达在肿瘤组织中普遍表达,NS基因可能参与了肿瘤细胞的增殖调控.
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| 关 键 词： | 肿瘤 基因表达 肿瘤增殖 |
SIGNIFICANCE OF NUCLEOSTEMIN GENE EXPRESSION IN TUMOUR TISSUES |
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| Zheng xuezhi et al.SIGNIFICANCE OF NUCLEOSTEMIN GENE EXPRESSION IN TUMOUR TISSUES[J].Journal of Mudanjiang Medical College,2008,29(2):3-5. |
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| Authors: | Zheng xuezhi |
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| Institution: | Zheng xuezhi et al (Mudanjiang Medical College Heilongjiang Mudanjiang 157011 |
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| Abstract: | Objective:To detect the NS gene expression in some tumour tissues and cells.Methods:We collected some human tumour tissues,then total RNA was isolated using kit;GAPDH gene was used as a loading control measured wether the NS gene expression in all tumuor tissues by RT-PCR.Results:We found that NS gene expressed in all the tumour tissues.Conclusion:NS gene expressed in tumour tissues was universality,the NS gene could participate in tumour proliferation. |
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| Keywords: | Nucleostemin |
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