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| 人子宫内膜腺上皮细胞及基质细胞分离方法的研究 | |
| 引用本文： | 王海燕,薛晓鸥,姜恩魁,戴俊峰.人子宫内膜腺上皮细胞及基质细胞分离方法的研究[J].辽宁医学院学报,2003,24(4):36-39. |
| 作者姓名： | 王海燕 薛晓鸥 姜恩魁 戴俊峰 |
| 作者单位： | 1. 锦州医学院生理学教研室 2. 锦州医学院附属第一医院妇产科 3. 锦州医学院附属第二医院口腔颌面外科,辽宁,锦州,121001 |
| 摘 要： | 目的 探索子宫内膜腺上皮细胞及基质细胞分离的新方法。方法 通过胶原酶阶梯消化(0．25％，0．15％，0．1％，0．08％)、不同目次的筛网过滤、重力沉降等技术得到高纯度的人子宫内膜腺上皮细胞和基质细胞。结果 应用浓度逐渐降低的胶原酶消化子宫内膜组织，可提高细胞的存活率及细胞收集量。筛网过滤和重力沉降法，可提高子宫内膜腺上皮细胞和基质细胞的纯度，腺上皮细胞可达90％，基质细胞可达96％以上。结论 采用此方法可得到大量高纯度的子宫内膜腺上皮细胞及基质细胞，为体外研究子宫内膜提供模型。 |
| 关 键 词： | 人子宫内膜 腺上皮细胞 基质细胞 分离 胶原酶 |
| 文章编号： | 1000-5161(2003)04-0036-04 |
| 修稿时间： | 2003年6月16日 |
Study on the Method of Isolation, Culture of Glandular Epithelial Cells and Stromal Cells of Human Endometrium by Collagenase-ladder Digest Assay |
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| WANG Hai-yan ,XUE Xiao-ou ,JINAG En-kui ,DAI Jun-feng.Study on the Method of Isolation, Culture of Glandular Epithelial Cells and Stromal Cells of Human Endometrium by Collagenase-ladder Digest Assay[J].Journal of Liaoning Medical University (LNMU) Bimonthly,2003,24(4):36-39. | |
| Authors: | WANG Hai-yan XUE Xiao-ou JINAG En-kui DAI Jun-feng |
| Institution: | WANG Hai-yan 1,XUE Xiao-ou 2,JINAG En-kui 1,DAI Jun-feng 2 |
| Abstract: | Objective To explore the new methods of isolating and culturing glandular epithelial cells and stromal cells of human endometrium.Methods Using the technique of different gradient(0 25%,0 15%,0 1%,0 08%)of collagenase,serial filtration and sedimentation,the higher purities of glandular epithelial cells and stromal cells were obtained.Results The yieds and the surviving rates of two kinds of cells were increased by collagenase-ladder digest assay.The purities of glandular epithelial cells and stromal cells enhanced,90% and 95% respectively,by serial filtration and sedimentation.Conclusions The high purities of glandular epithelial cells and the stromal cells can be used as one of the cell models in vitro by those techniques. |
| Keywords: | human endometrium glandular epithelial cell stromal cell collagenase |
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