肺癌中医证候与代谢酶基因CYP1A1、CYP2E1多态性的关联研究

目的:探讨代谢酶基因多态性与肺癌中医证型的关联,寻找肺癌中医证型的易感基因,分析基因辅助辨证的可行性。方法:分析207例肺癌患者的中医证型,运用聚合酶链反应-连接酶检测(polymerase chain reaction-ligase detection reaction,PCR-LDR)技术检测207例肺癌患者CYP1A1基因MspⅠ、Ⅱe/val位点和CYP2E1基因RsaI位点的基因型,分析其基因型和等位基因频率分布与肺癌中医证候之间的关系。结果:CYP1A1基因MspⅠ位点3种基因型在不同中医证型中的分布具有统计学差异(P0.01);精气两亏证CC基因型和C等位基因出现的频率明显高于脾虚痰湿证和痰热内蕴证(P0.0083);痰热内蕴证以CT基因型为主(50.0%),阴虚内热证以CC基因型为主(76.2%),两组具有显著差异(P0.0083)。CYP1A1基因Ⅱe/val位点和CYP2E1基因RsaI位点基因型及等位基因频率在4组证型中的分布,差异均无统计学意义(P0.05)。结论:CYP1A1基因MspⅠ位点可能与肺癌中医证型具有关联,CC基因型和C等位基因可能与精气两亏证的形成相关联。

Correlation Between TCM Syndrome and Genetic Polymorphism of CYP1A1 and CYP2E1 in Patients with Lung Cancer

Objective：To investigate the relationship between genetic polymorphism and traditional Chinese medicine（TCM） syndrome and search for the susceptible gene of TCM syndrome in patients with lung cancer. Methods： TCM syndromes of 207 lung cancer patients were differentiated. The genotypes of MspⅠ,Ⅱe/val in CYP1A1 gene and RsaI in CYP2E1 gene were detected by polymerase chain reaction-ligase detection reaction （PCR-LDR） in 207 lung cancer patients. The relationship between genotype and allelic distribution and TCM syndrome of lung cancer was analyzed. Results： There were statistical differences in genotypes of MspⅠin CYP1A1 gene among different TCM syndrome types（P0.01）. The frequencies of CC genotype and C allele in syndrome of essence and qi deficiency were higher than that in syndrome of spleen-deficiency and phlegm-dampness and syndrome of internal accumulation of phlegm-heat （P0.0083）. There were significant differences between syndrome of internal accumulation of phlegm-heat in which CT was the main genotype（50.0%）and syndrome of internal heat due to yin-deficiency in which CC was the main genotype（76.2%）（P0.0083）. There were no statistical differences in genotypes and allelic distributions of Ⅱe/val in CYP1A1 gene and RsaI in CYP2E1 gene among different TCM syndrome types （P0.05）. Conclusion： There may be relationship between the genetic polymorphism of MspⅠin CYP1A1 gene and TCM syndrome of lung cancer. CC genotype and C allele may be related to the formation of syndrome of essence and qi deficiency.