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调宁蛋白在胆固醇结石形成中的表达及养肝柔肝中药的干预作用
引用本文:高炬,方邦江,朱培庭,张静喆,张奕缨,裴新军,李炯,张丽朵,陈宝瑾,梁晓强,王佑华.调宁蛋白在胆固醇结石形成中的表达及养肝柔肝中药的干预作用[J].上海中医药大学学报,2008,23(3):55-57.
作者姓名:高炬  方邦江  朱培庭  张静喆  张奕缨  裴新军  李炯  张丽朵  陈宝瑾  梁晓强  王佑华
作者单位:1. 上海中医药大学附属龙华医院胆道外科,上海,200032
2. 上海中医药大学附属龙华医院急诊科,上海,200032
基金项目:国家自然科学基金 , 上海市重点学科建设项目 , 全国急诊临床基地建设项目
摘    要:目的:观察实验豚鼠胆囊组织调宁蛋白(Cap)表达水平的变化.探讨养肝柔肝中药防治胆石病的作用机制。方法:将受试豚鼠随机分为4组,即正常组、模型组、养肝柔肝中药组(中药组)、熊去氧胆酸西药组(西药组)。后3组采用高胆固醇致石食饵诱发法建立豚鼠胆结石动物模型,其中中药组、西药组在造模的同时分别给予相应药物干预;连续7周后,分别对实验豚鼠胆结石生成情况,胆囊组织Cap蛋白的表达进行观察。结果:养肝柔肝中药组能有效防止胆结石的生成,与模型组比较有显著性差异(P〈0.01),并且其效果优于熊去氧胆酸西药组(P〈0.05)。模型组动物胆囊组织CaD蛋白表达水平较正常组显著升高(P〈0.01);养肝柔肝中药组和熊去氧胆酸西药组胆囊组织Cap蛋白表达水平虽均高于正常组(分别为P〈0.05,P〈0.01),但较模型组明显降低(均P〈0.01),且养肝柔肝中药组Cap蛋白表达水平较低,但与熊去氧胆酸西药组无显著性差异(P〉0.05)。结论:养肝柔肝中药能显著降低胆结石的形成,其作用机制可能与下调胆囊平滑肌组织Cap蛋白的水平,提高胆囊平滑肌收缩能力有关。

关 键 词:胆结石  养肝利胆颗粒剂  胆固醇  调宁蛋白

Calponin Expression in Cholesterol Gallstone Formation and Its Intervention by Liver-Nourishing and Liver-Softening Herbs
GAO Ju,FANG Bang-jiang,ZHU Pei-ting,ZHANG Jing-zhe,ZHANG Yi-ying,PEI Xin-jun,LI Jiong,ZHANG Li-duo,CHEN Bao-jin,LIANG Xiao-qiang,WANG You-hua.Calponin Expression in Cholesterol Gallstone Formation and Its Intervention by Liver-Nourishing and Liver-Softening Herbs[J].Acta Universitatis Traditionis Medicalis Sinensis Pharmacologiaeque Shanghai,2008,23(3):55-57.
Authors:GAO Ju  FANG Bang-jiang  ZHU Pei-ting  ZHANG Jing-zhe  ZHANG Yi-ying  PEI Xin-jun  LI Jiong  ZHANG Li-duo  CHEN Bao-jin  LIANG Xiao-qiang  WANG You-hua
Institution:GAO Ju FANG Bang-jiang ZHU Pei-ting ZHANG Jing-zhe ZHANG Yi-ying PEI Xin-jun LI Jiong ZHANG Li-duo CHEN Bao-jin LIANG Xiao-qiang WANG You-hua (Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine)
Abstract:Objective: To observe the expression of Calpenin in the formation of cholesterol gallstone and explore the mechanisms of liver-nourishing and liver-softening herbs in preventing and treating gallstone. Methods: The guinea pigs were randomly divided into 4 groups: normal group, model group, herb group and control group. The guinea pigs of the latter 3 groups were modeled gallstones by high-cholesterol diet. Liver-nourishing and liver-soften- ing herbs and ursodeoxycholic acid were given to the guinea pigs in herb and control groups respectively for seven weeks. The gallstone formation and Calpenin expression in gallbladder were observed. Results: The herbs could prevent the formation of gallstone significantly (P 〈 0.01), with more significant effects than ursodeoxycholic acid(P 〈 0.01). The Calpenin expression in model group was significantly increased as compared with that in normal control (P 〈 0.01). The Calponin expressions in herb group or control group were increased as compared with that in normal group (P 〈 0.05, P 〈 0.01), but they were decreased as compared with that in model group (P 〈 0.01). There was no significant difference in the Calpenin expression between herb group and control group (P 〉 0.05). Conclusion: Liver-nourishing and liver-softening herbs can decrease the gallstone formation significantly possibly by down-regulating the Calpenin expression in the smooth muscle of gallbladder and enhancing the contractibility of smooth muscle in gallbladder.
Keywords:Gallstones  liver-nourishing and liver-soitening granule  cholesterol  IXalpomn
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