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| 四妙勇安汤的有效成分对血管内皮细胞增殖的影响 | |
| 引用本文： | 袁卓,张军平,张仁岗.四妙勇安汤的有效成分对血管内皮细胞增殖的影响[J].上海中医药大学学报,2008,22(4):69-71. |
| 作者姓名： | 袁卓 张军平 张仁岗 |
| 作者单位： | 1. 天津中医药大学,天津,300193 2. 天津中医药大学第一附属医院,天津,300193 3. 天津中医药大学,天津,300193;安徽中医学院,安徽,合肥,230038 |
| 基金项目： | 国家自然科学基金，教育部跨世纪优秀人才培养计划，高等学校博士学科点专项科研项目 |
| 摘 要： | 目的：研究四妙勇安汤的有效成分对脐静脉内皮细胞（ECV304）增殖的影响,以探讨其促血管新生的可能机制。方法：体外培养ECV304,单体进行干预,利用MTT及BrdU-ELISA法检测其对ECV304增殖的影响。结果：绿原酸10^1ng/ml-10^2ng/ml,阿魏酸10^2ng/ml-10^4ng/ml浓度组为促细胞增殖的优选浓度。结论：绿原酸、阿魏酸促内皮细胞增殖能力与血清有协同作用。 |
| 关 键 词： | 四妙勇安汤 内皮细胞 增殖 绿原酸 阿魏酸 |
Influences of Effective Components in "Simiao Yong an Decoction" on Proliferation of Vascular Endothelial Cells in vitro |
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| YUAN Zhuo,ZHANG Jun-ping,ZHANG Ren-gang.Influences of Effective Components in "Simiao Yong an Decoction" on Proliferation of Vascular Endothelial Cells in vitro[J].Acta Universitatis Traditionis Medicalis Sinensis Pharmacologiaeque Shanghai,2008,22(4):69-71. | |
| Authors: | YUAN Zhuo ZHANG Jun-ping ZHANG Ren-gang |
| Institution: | YUAN Zhuo ZHANG Jun-ping ZHANG Ren-gang(1. Tianjin University of Traditional Chinese Medicine;2. No.1 Hospital Affiliated to Tianjin University of Traditional Chinese Medicine;3. Anhui College of Traditional Chinese Medicine) |
| Abstract: | Objective： To investigate the influence of effective components in ＂Simiao Yong an Decoction＂ on proliferation of umbilical vein vascular endothelial cells ECV304 in vitro and its possible mechanism of promoting angiogenesis. Methods： ,In vitro culture, the influences of medicinal monomer of ＂Simiao Yong＇an Decoction＂ on proliferation of ECV304 were detected by means of MTT and BrdU-ELISA. Results： The optimal concentration of chlorogenic acid in promoting cell proliferation was 10^1ng/ml-10^2ng/ml,and that of ferulic acid was 10^2ng/ml-10^4ng/ml.Conclusion： Chlorogenic acid and ferulic acid can promote endothelial cell proliferation in a cooperative relation with fetal bovine serum （FBS）. |
| Keywords: | ＂Simiao Yong an Decoction＂ vascular endothelial cells proliferation cholorogenic acid fenulic acid |
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