| 蜂毒素对骨肉瘤细胞系细胞增殖的影响 |
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| 引用本文： | 陈永强,朱振安,戴克戎,张晨.蜂毒素对骨肉瘤细胞系细胞增殖的影响[J].上海中医药大学学报,2003,17(1):55-57. |
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| 作者姓名： | 陈永强 朱振安 戴克戎 张晨 |
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| 作者单位： | 1. 上海第二医科大学附属第九人民医院骨科,上海,200011
2. 第二军医大学附属长海医院中医科实验室,上海,200433 |
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| 摘 要： | 为了解蜂毒素对骨肉瘤细胞生长、增殖的作用。以骨肉瘤U2OS细胞体外培养，采用台盘蓝排染法观察蜂毒素对细胞生长的影响，MTT法测定细胞增殖抑制率，流式细胞仪检测细胞周期和增殖细胞核抗原（PCNA）的表达。结果：蜂毒素可明显抑制U2OS细胞生长、增殖，下调PCNA的表达，对细胞周期的影响表现为S期细胞的阻滞。提示蜂毒素具有抑制骨肉瘤U2OS细胞株生长、增殖的作用。
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| 关 键 词： | 蜂毒素 骨肉瘤 增殖 中医药疗法 |
| 文章编号： | 1008-861X(2003)01-0055-03 |
| 修稿时间： | 2003年1月8日 |
Influence of Bee Venom on Cell Proliferation of Osteosarcoma Cells |
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| CHEN Yong qiang,ZHU Zhen an,DAI Ke rong No. People's Hospital Affiliated to Shanghai Second Medical University ZHANG Chen Institute of Chinese and Western Medicine Integration,Chang Hai Hospital of Second Military Hospital.Influence of Bee Venom on Cell Proliferation of Osteosarcoma Cells[J].Acta Universitatis Traditionis Medicalis Sinensis Pharmacologiaeque Shanghai,2003,17(1):55-57. |
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| Authors: | CHEN Yong qiang ZHU Zhen an DAI Ke rong No People's Hospital Affiliated to Shanghai Second Medical University ZHANG Chen Institute of Chinese and Western Medicine Integration Chang Hai Hospital of Second Military Hospital |
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| Institution: | CHEN Yong qiang,ZHU Zhen an,DAI Ke rong No.9 People's Hospital Affiliated to Shanghai Second Medical University ZHANG Chen Institute of Chinese and Western Medicine Integration,Chang Hai Hospital of Second Military Hospital |
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| Abstract: | In order to understand the function of bee venom on the growth and proliferation of osteosarcoma cells, by culture in vitro of U 2 OS cells, the trypan blue stain was used to observe influence of bee venom on cell growth. MTT method was used to determine the inhibiting rate of cell proliferation. And the flow type cell apparatus was used to determine the cell cycle and the expression of proliferated cell nucleolus antigen (PCNA). The results showed that bee venom could obviously inhibit the growth and proliferation of U 2 OS cells and decrease PCNA expression, with influence on cell cycle manifested as a blockage of phase C cells. The findings indicate that bee venom has the functions to inhibit the growth and proliferation of U 2 OS cell strain. |
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| Keywords: | Bee venom osteosarcoma proliferation |
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