健脾清化方对不同蛋白饲料喂养慢性肾衰竭大鼠肾功能及血脂的影响

目的：观察健脾清化方对不同蛋白饲料喂养慢性肾衰竭大鼠肾功能及血脂的影响。方法：以5/6肾切除法及不同蛋白饲料喂养,建立3种不同蛋白饲料喂养的慢性肾衰竭大鼠模型,以蒸馏水、健脾清化方和蒙诺灌胃干预8周。干预结束后,检测不同组别大鼠24h尿蛋白定量及血尿素氮（BUN）、肌酐（Scr）、胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白（HDL）和低密度脂蛋白（LDL）的水平。结果：各模型组大鼠24h尿蛋白定量及BUN、Scr、TC、TG、HDL和LDL水平均较正常组明显升高;应用高脂饲料两种模型组的TC、TG、HDL及LDL明显高于正常饲料模型组。健脾清化方能显著降低各模型24h尿蛋白定量和高脂饲料两种模型Scr、TG、TC、LDL。结论：高脂饮食可以加重慢性肾衰竭大鼠的脂质代谢异常;健脾清化方可减少慢性肾衰竭高脂血症大鼠24h尿蛋白定量,改善其肾功能,其作用机制可能与改善脂质代谢紊乱相关。

Effects of"Jianpi Qinghua Decoction"on Renal Function and Blood Lipid in Chronic Renal Failure Rats by Different Fat Feeds

Objective:To observe the effects of "Jianpi Qinghua Decoction" on renal function and blood lipid in chronic renal failure(CRF) rats by different fat feeds. Methods:Three CRF rat models were establishes by undergoing 5/6 nephrectomy and supplying different fat feeds. After eight weeks of treatment with distilled water,"Jianpi Qinghua Decoction"and Monopril,the levels of 24-h urine protein(Upr),blood urea nitrogen(BUN)and serum creatinine(Cr),total cholesterol(TC),triglycerides(TG),high-density lipoprotein(HDL)and low-density lipoprotein(LDL)of rats in these three models were examined. Results:The levels of 24-h Upr,BUN and serum Cr,TC,TG and LDL in the three models were significantly higher than those in the untreated groups. The levels of TC,TG,HDL and LDL of two models by supplying high-fat feeds were significantly higher than the model by supplying normal-fat feed. The levels of 24-h Upr in Decoction-treated groups of three models were significantly lower than those in the untreated group of three models. The levels of Scr,TG,TC and LDL in Decoction-treated groups of two models by supplying high-fat feed were significantly lower than those in the untreated groups of two models by supplying high-fat feed. Conclusion:The results indicate that supplying high-fat feed can worsen the lipid metabolism of rats with chronic renal failure,and "Jianpi Qinghua Decoction" can improve the renal function and hyperlipidemia by regulating lipid metabolism.