白细胞介素12基因修饰的Lewis肺癌瘤苗的抗肿瘤免疫作用

目的：研究白细胞介素12（IL-12）基因修饰的Lewis肺癌瘤苗的抗肿瘤免疫作用。方法：用含IL-12基因的腺病毒载体，在体外以不同感染系数感染Lewis肺癌细胞，并分别接种于C57BL/6小鼠皮下，观察其致瘤性的变化；并用Lewis肺癌细胞及B16黑色素瘤细胞再 次攻击免疫后的C57BL/6小鼠，观察肿瘤发生情况。 结果：以各种感染系数AdCMVIL-12制备的瘤苗，接种于小鼠皮下后，无肿瘤生长，完全消除了Lewis肺癌细胞的致瘤性；瘤苗注射6 d后，用Lewis肺癌细胞再次攻击，仍无肿瘤生长，抑瘤率达100%；用B16黑色素瘤细胞攻击，10~12 d均有肿瘤生长，说明此瘤苗具有肿瘤特异性。结论：AdCMVIL-12能消除Lewis肺癌细胞的致瘤性，并能诱导显著的抗肿瘤免疫反应，对肿 瘤的复发及转移有较好疗效。

Antitumor immune activities of Lewis lung cancer cells modified by interleukin-12 gene

Objective To study the antitumor immune activities of the Lewis lung cancer cells modified by interleukin-12(IL-12) gene in vivo.Methods The Lewis lung cancer cells were transfected with AdCMIL-12 by different multiplicity of infection(MOI).C57BL/6 mice were treated by subcutaneous vaccination with the differently disposed Lewis lung cancer cells,and then challenged by Lewis lung cancer cells and B16 melanoma,respectively.The tumor growth was observed.Results The tumor growth in the vaccine group was inhibited significantly,not only when C57BL/6 mice were treated with the differently disposed Lewis lung cancer cells,but also when C57BL/6 mice were challenged again by Lewis lung cancer cells after subcutaneous vaccination for 6 d.But the tumors occurred when C57BL/6 mice were challenged again by B16 melanoma.These results indicated that the tumor vaccine had the tumor specificity.Conclusion Immunization with the Lewis lung cancer cells transfected by AdCMIL-12 could induce potent antitumor immune activity specifically,and may have significant therapeutic effects on the recurrence and the metastasis of lung cancer.