| 人参三醇组皂甙对大鼠心室肌细胞L型、T型、B型钙通道的阻滞作用 |
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| 引用本文： | 王晓明,江岩,王中峰,钟国赣,张文杰,马兴元,安占元.人参三醇组皂甙对大鼠心室肌细胞L型、T型、B型钙通道的阻滞作用[J].吉林大学学报(医学版),1993(2). |
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| 作者姓名： | 王晓明 江岩 王中峰 钟国赣 张文杰 马兴元 安占元 |
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| 作者单位： | 白求恩医科大学生理中心实验室
(王晓明，江岩，王中峰)，白求恩医科大学生理教研室
(钟国赣，张文杰)，白求恩医科大学有机化学教研室
(马兴元)，白求恩医科大学有机化学教研室(安占元) |
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| 摘 要： | 分离Wistar大鼠乳鼠的心室肌细胞,向培养基中分别加入人参三醇组皂甙300μg/ml,钙通道阻滞剂异博定37.5μg/ml或钙通道激动剂BAY K 8644 5μmol/L。用斑片钳的连细胞电压钳法,记录加药前后L、T、B三型钙通道的单通道活动。确证了人参三醇组皂甙的钙通道阻滞作用,并揭示其作用机理在于使钙通道的开放时间缩短与开放概率减少。
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| 关 键 词： | 人参三醇组皂甙 培养的心肌细胞 斑片钳 钙通道 BAY K 8644 异博定 |
CALCIUM CHANNEL BLOCKADE ACTION OF PANAXATRIOL SAPONINS ON THE L TYPE, T TYPE AND B TYPE CALCIUM CHANNELS OF CULTURED RAT CARDIOMYOCYTES |
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| Wang Xiaoming,Zhong Guogan,Ma Xingyuan,Jiang Yan,Zhang Wenjie,An Zhanyuan,Wang Zhongfeng.CALCIUM CHANNEL BLOCKADE ACTION OF PANAXATRIOL SAPONINS ON THE L TYPE, T TYPE AND B TYPE CALCIUM CHANNELS OF CULTURED RAT CARDIOMYOCYTES[J].Journal of Jilin University: Med Ed,1993(2). |
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| Authors: | Wang Xiaoming Zhong Guogan Ma Xingyuan Jiang Yan Zhang Wenjie An Zhanyuan Wang Zhongfeng |
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| Abstract: | Wistar rat ventricular myocardiocytes were isolated. Panaxatriol saponins (PTS) 300μg/ml, calcium channel blocker verapamil 37. 5μg/ml or calcium channel activator BAY K 8644 5μmmol/L were added into the bath solution separately. The single channel activities of L, T and B type calcium channels were recorded before and after administration, using the voltage patch-clamp technique in cell-attached configuration. The calcium channel blockade effect of PTS was exactly demonstrated. It was disclosed that the mechanism involved decrease in both the open time and the open-state probability of the calcium channel. |
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| Keywords: | Panaxatriol saponins Cultured myocardiocytes Patch-clamp Calcium channel BAY K 8644 Verapamil |
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