五味子乙素对脑胶质瘤SHG-44细胞VEGF表达水平的影响及其细胞生长抑制作用

目的：探讨五味子乙素（Sch B）作用于脑胶质瘤SHG-44细胞后对血管内皮生长因子(VEGF)表达的影响及细胞生长抑制作用，阐明其可能机制。方法：培养脑胶质瘤SHG-44细胞，将其分为对照组和Sch B　50、100、200 mg?L^-1组，MTT法检测不同剂量Sch B作用SHG-44细胞后其生长情况；酶联免疫吸附实验和细胞免疫组织化学方法观察Sch B作用后SHG-44细胞VEGF蛋白表达情况。结果：与对照组比较，200 mg?L^-1Sch B作用SHG-44细胞24 h，细胞增殖活性轻度增加（P<0.05）;Sch B （50、100 和200 mg?L^-1）作用48或72 h，细胞增殖活性明显降低（P<0.05或P<0.01）；200 mg?L^-1Sch B作用SHG-44细胞72 h，培养上清中VEGF蛋白表达水平明显降低(P<0.01）。与对照组比较，Sch B作用SHG-44细胞72 h后，Sch B 50、100和200 mg?L^-1组VEGF蛋白阳性表达率均明显降低（P<0.01）。结论：Sch B能明显抑制SHG-44细胞VEGF蛋白表达及细胞生长，提示Sch B可能通过抑制脑胶质瘤血管生成而发挥抗肿瘤作用。

Effect of schisandrin B on expression of vascular endothelial growth factor in glioma SHG-44 cells and its inhibitory effect on cell growth

Objective To explore the effect of schisandrin B(Sch B) on expression of vascular endothelial growth factor(VEGF) in glioma SHG-44 cells and its inhibitory effect on cell growth and to explore the possible mechanism.Methods Glioma SHG-44 cells were separated to control and 3 treatment groups,and then treated with 0,50,100 and 200 mg·L-1 Sch B for 24-72 h.MTT assay was used to evaluate the proliferation of glioma SHG-44 cells.The levels of VEGF protein expression after treated with Sch B were measured by both ELISA and immuncytochemistry.Results Compared with control group,the proliferation activity of glioma SHG-44 cells was slightly increased after treated with 200 mg·L-1 Sch B for 24 h(P<0.05);the proliferation abilities of the SHG-44 cells were obviously decreased after treated with 50,100 and 200 mg·L-1 Sch B for 48 h or 72 h;the VEGF protein level in the supernatant of cultured cells after treated with Sch B for 72 h was significantly decreased(P<0.01).After the glioma SHG-44 cells were treated with Sch B for 72 h,the positive expression rates of VEGF protein in 50,100 and 200 mg·L-1 groups were markedly reduced compared with control group(P<0.01).Conclusion Sch B can inhibit the expression of VEGF protein and growth of glioma SHG-44 cells,it suggests that Sch B may exert anti-glioma effect by inhibiting angiogenesis in glioma.