下调ADAR1表达对人肺癌细胞增殖、迁移和上皮间质转化的影响

目的：探讨下调作用于RNA的腺苷酸脱氢酶1（ADAR1）对肺癌H1299和H520细胞增殖、迁移和上皮间质转化（EMT）的影响，并阐明其作用机制。方法：H1299和H520细胞系分为对照组（转染negative shRNA）和shADAR1组（转染shADAR1）。实时荧光定量PCR法和Western blotting法检测各组细胞中ADAR1 mRNA和蛋白表达水平，CCK-8法和克隆形成实验检测各组细胞增殖活性和克隆形成数，细胞划痕实验检测各组细胞划痕愈合率，Western blotting法检测各组细胞中E钙黏附蛋白（E-cadherin）和波形蛋白（vimentin）蛋白表达水平。结果：与对照组比较，shADAR1组的H1299和H520细胞中ADAR1 mRNA及蛋白表达水平均明显降低（P<0.05或P<0.01）。与对照组比较，shADAR1组H1299和H520细胞增殖活性、克隆形成数量和细胞划痕愈合率均明显降低（P<0.05或P<0.01），H1299和H520细胞中E-cadherin蛋白表达水平明显升高（P<0.01），vimentin蛋白表达水平明显降低（P<0.01）。结论：下调ADAR1表达能够抑制肺癌细胞的增殖、迁移及EMT。

Effects of down-regulation of ADAR1 expression on proliferation,migration and epithelial-mesenchymal transition of human lung cancer cells

Objective: To investigate the effects of down-regulation of adenosine deaminase acting on RNA enzyme 1 (ADAR1) on the proliferation, migration and epithelial-mesenchymal transition (EMT) of the lung cancer H1299 and H520 cells, and to elucidate their mechanisms. Methods: The H1299 and H520 cells were divided into control group (transfected with negative shRNA) and shADAR1 group (transfected with shADAR1). Real-time fluorescence quantitative PCR and Western blotting methods were used to detect the expression levels of ADAR1 mRNA and protein, respectively. CCK8 method and colony information assay were used to detect the proliferation activities and the amounts of colony formation;scratch assay was used to detect the scratch healing rates of the cells in various groups; Western blotting method was performed to detect the expression levels of E-cadherin and vimentin proteins in the cells in various groups. Results: Compared with control group, the expression levels of ADAR1 mRNA and protein in the H1299 and H520 cells in shADAR1 group were significantly decreased(P<0.05).Compared with control group, the proliferation activity, the colony formation amount and the scratch healing rate of the H1299 and H520 cells in shADAR1 group were significantly decreased (P<0.05).Compared with control group, the expression levels of E-cadherin protein in the H1299 and H520 cells in shADAR1 group were significantly increased(P<0.01), and the expression levels of vimentin protein were decreased (P<0.01). Conclusion: Down-regulation of ADAR1 expression contributes to inhibiting the proliferation, migration, and EMT of lung cancer cells.