松茸多糖对X射线照射小鼠造血功能的保护作用

目的：探讨松茸多糖(PTM)对辐射损伤小鼠造血功能的保护作用。方法：将50只ICR小鼠随机分成5组,每组10只。正常对照组(NC)和辐射对照组(IC)给予生理盐水灌胃，3个剂量PTM组分别给予400、800和1 200 mg?kg^-1剂量PTM灌胃，每天1次，连续7 d。第8天给予IC组和PTM组动物进行全身一次性照射，总剂量2.0 Gy，检测小鼠造血干细胞集落形成单位（CFU-S）数 、骨髓基质细胞-成纤维祖细胞集落形成单位（CFU-F）数、骨髓细胞周期和骨髓微核率。结果：与IC组比较，预防给予PTM各组小鼠CFU-S数量明显减少( P<0.05或 P<0.01)、骨髓CFU-F数显著升高( P<0.05或 P<0.01)，中、高剂量PTM组PCE微核的数量明显降低(P<0.05或 P<0.01)，G₁期细胞数明显减少( P<0.05或 P<0.01)，S期细胞数明显增加( P<0.05或 P<0.01)。结论：松茸多糖对辐射所致的造血损伤有明显的保护作用。

Protective effect of polysaccharides of tricholoma matsutake on hematopoietic function of X-rays irradiated mice

Objective To explore the protective effect of the polysaccharides of tricholoma matsutake(PTM) on hematopoietic function of X-rays irradiated mice.Methods Fifty ICR mice were divided into five groups randomly,10 each group.The mice in normal control group(NC) and radiation control group(IC) were gaster-poured by 0.9% sodium chloride solution.The mice in PTM groups were gaster-poured by three different doses PTM(400,800,1200 mg·kg-1) for 7 d.The mice all groups except NC group were irradiated once with 2.0 Gy X-rays on the eighth day.The conony forming units-spleen(CFU-S),fibrolast colony forming unit(CFU-F),cell cycle of bone marrow cells and micronucleus rate of bone marrow polychromalic erythrocytes(PCE) were detected after radiation for 24 h.Results Compared with IC group,the number of CFU-S in PTM groups decreased significantly(P<0.05 or P<0.01)and the number of CFU-F increased markedly(P<0.05 or P<0.01);in high and moderate doses PTM the micronucleus rates of bone marrow PCE and the percentage of bone marrow cells at G1 phase decreased signficantly(P<0.05 or P<0.01);the percentage of cells at S phase increased signficantly(P<0.05 or P<0.01).Conclusion PTM has an obvious protective effect on hematopoietic function of X-rays irradiated mice.